TY - JOUR AU - Bualuang, Aporn AU - Boonburapong, Bongkoj AU - Laloknam, Surasak PY - 2022/08/30 Y2 - 2024/03/29 TI - Optimization of Arthrospira platensis growth using organic culture medium JF - Asia-Pacific Journal of Science and Technology JA - Asia Pac J Sci Technol VL - 27 IS - 05 SE - ICoFAB 2021 Articles DO - 10.14456/apst.2022.68 UR - https://so01.tci-thaijo.org/index.php/APST/article/view/261616 SP - APST-27-05-03 (9 pages) AB - <p>In this study, an organic culture medium for <em>Arthrospira platensis</em> (Spirulina) was created using sugarcane molasses (SGCM), soybean meal (SBM), and small fish bone meal (SFBM) as substitutions for macronutrients and micronutrients in Zarrouk’s medium. Cell growth of <em>A. platensis</em> was monitored using spectrophotometric analysis. The growth patterns of <em>A. platensis</em> under normal and lack of carbon, nitrogen, and micronutrient conditions showed that the optimal growth of a lack of carbon, nitrogen, and micronutrient was decreased by about 50% compared to normal conditions. A singular material was designed to substitute macronutrients and micronutrients in Zarrouk’s medium. In the SGCM medium, the total reducing sugars were varied by concentration in the range of 0 to 3.0% (w/v), while the total protein in the SBM medium was similar. The percentage of dried SFBM in the SFBM medium was also in the concentration range of 0 to 1.0 % (w/v). The results showed that the optimum concentrations of the SGCM, SBM and SFBM media were 0.2% (w/v), 0.5% (w/v) and 0.1% (w/v), respectively. An organic combination medium was designed using optimal concentrations of SGCM, SBM, and SFBM. For a period of 30 days, cell growth of <em>A. platensis </em>was studied and compared to Zarrouk's medium. The wet weights of <em>A. platensis</em> in the combination medium and Zarrouk's medium were 31.25 g and 33.46 g, respectively. The findings indicated that the combination-medium composed of organic substance medium could be used successfully as a culture medium for <em>A. platensis</em> within organic system.</p> ER -