Asia-Pacific Journal of Science and Technology
https://so01.tci-thaijo.org/index.php/APST
<p style="text-align: justify;"><strong><a title="ANNOUNCEMENT Effective May 1, 2025, APST will begin using our new website. Please access the new platform at https://apst.kku.ac.th Thank you for your cooperation during this transition." href="https://apst.kku.ac.th" target="_blank" rel="noopener">ANNOUNCEMENT</a><br /><a title="ANNOUNCEMENT Effective May 1, 2025, APST will begin using our new website. Please access the new platform at https://apst.kku.ac.th Thank you for your cooperation during this transition." href="https://apst.kku.ac.th" target="_blank" rel="noopener">Effective May 1, 2025, APST will begin using our new website. Please access the new platform at https://apst.kku.ac.th Thank you for your cooperation during this transition.</a><br /><br />Asia-Pacific Journal of Science and Technology (APST)</strong></p> <p style="text-indent: 2.5em;">The Asia-Pacific Journal of Science and Technology (APST), formerly known as the KKU Research Journal, is a peer-reviewed journal published by the Research and Technology Transfer Affairs Division of Khon Kaen University. The journal's title was changed in order to attract more international readers and authors.</p> <p style="text-indent: 2.5em;">The journal accepts English-language manuscripts of original research and review articles which have never been published elsewhere. The main focus of the journal is to publish work that leads to creative practical applications, environment preservation, and human well-being. Research areas covered by the journal include engineering science, technology, agricultural science, and health science. Manuscripts reporting on research that integrates any of the aforementioned fields are especially encouraged. Our core interest is in the use of engineering, scientific, and technological knowledge to assist, co-operate with, and enhance other fields, such as health and agricultural science, in order to improve human well-being. We particularly welcome research that integrates various fields, including plant science and pharmaceutical science or plant science, agricultural engineering, automatic control, and energy technology.</p> <p style="text-indent: 2.5em;">The journal employs a double-blind review process, in which authors and reviewers are anonymous to each other throughout the process. </p> <p><strong>Journal Abbreviation</strong>: Asia Pac J Sci Technol<br /><strong>Online ISSN</strong>: 2539-6293<br /><strong>Start Year</strong>: 1996<br /><strong>Language</strong>: English (since Vol. 20 No. 1, 2015)<br /><strong>Publication Fee</strong>: Free<br /><strong>Issues per Year</strong>: 6 Issues (start Vol. 27 No. 1, 2022)</p> <p><a title="https://so01.tci-thaijo.org/index.php/APST/announcement" href="https://so01.tci-thaijo.org/index.php/APST/announcement"><strong>Notification of changes in the submission system</strong></a></p> <p style="text-align: justify;">Dear Authors of APST, please be informed that from December 9th, 2022, onward, the journal will change its submission and reviewing platform from the Editorial Manager (EM) to the Thailand Editorial System (Thai ES). For manuscripts submitted via EM system before December 9th, 2022, the review will continue to be carried out using the Editorial Manager (EM) until the process is completed.</p> <p><a href="https://apst.kku.ac.th/wp-content/uploads/2025/09/Updated-APST-format-September-16-2025.docx"><strong>Download Template Guidelines Here</strong></a></p> <p> </p>Khon Kaen Universityen-USAsia-Pacific Journal of Science and Technology2539-6293Advancing sustainable agriculture through beneficial microbes: From microbial physiology to synthetic community-based innovations
https://so01.tci-thaijo.org/index.php/APST/article/view/283748
<p>This is a review of the use of effective microorganisms (EM) in sustainable agriculture to improve soil quality, increase crop yields, and control plant diseases. This review aims to contribute to understanding sustainable agriculture using microbes for our benefit by integrating microbial physiology and synthetic microbial innovations, with respect to community-level microbial interactions. Using a literature search strategy, the physiological mechanisms by which EM may enhance soil fertility through improved nutrient cycling and increased nutrient availability to plants, and/or by creating or modifying soil structure to support root growth, were described. Results showed that EM also increase the ability of plants to grow despite biotic stressors, such as soil-borne pathogens, through both the creation of antimicrobial compounds and competitive exclusion. Microbes also enhance plant tolerance to abiotic stressors, such as water loss from drought or salt accumulation, by altering how plants respond physiologically and by retaining soil moisture. This could be achieved by using effective microbes in agriculture via synthetic microbial communities (SynCom), which will enable greater resource efficiency, a lower environmental footprint, and contribute to achieving food security while addressing current global issues such as climate change and growing populations. However, there is still much to be learned about the long-term ecological implications, scalability, regulatory environments, and socio-economic feasibility of agricultural technologies based on microorganisms and SynComs, especially in developing and climate-vulnerable countries. Therefore, a comprehensive review that links microbial physiology, SynCom design, empirical data from crop studies, and future paths to innovation is both timely and needed.</p>Jomar L. AbanAllen Dale L. de GuzmanMark Joseph B. EscobalKaren Nicole F. EstalNathanael Alexis S. MilanesAnalyn V. Sagun
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2026-05-202026-05-20310331-03-04 (11 pages)10.22299/apst.2026.283748Influence of Host Matrix on Optical Properties of Samarium-Doped Glasses: A Comparative Analysis of Zinc Oxide and Zinc Fluoride
https://so01.tci-thaijo.org/index.php/APST/article/view/281539
<p>Samarium-doped glasses have attracted considerable interest due to their promising optical properties for use in photonic, laser, and optoelectronic applications. In this work, a comparative study was conducted on Sm³⁺-doped borotellurite glasses with the chemical composition 29TeO₂–30B₂O₃–10X–20BaO–10Nb₂O₅–1Sm₂O₃, where X represents either ZnO or ZnF₂. The influence of the modifier type (oxide vs. fluoride) on the structural and luminescence behavior of the glasses was thoroughly examined. The incorporation of Sm³⁺ ions into these host matrices leads to significant variations in optical performance, dictated by the local environment and bonding characteristics. Comprehensive characterization, including absorption and emission spectra, and luminescence lifetimes, was performed to evaluate the suitability of each glass system for optical device applications. The findings highlight the critical role of glass composition in tailoring the emission efficiency and optical response of rare-earth-doped glass materials.</p>Wiraphat ThanyaphirakPatarawagee YasakaKitipun BooninJakrapong Kaewkhao
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2026-05-202026-05-20310331-03-01 (10 pages)10.22299/apst.2026.281539Growing Miniature Waterlilies Under Different Lighting Conditions
https://so01.tci-thaijo.org/index.php/APST/article/view/273811
<p>An experiment was conducted at the Faculty of Agriculture and Natural Resources, Rajamangala University of Technology Tawan–ok, Chonburi, from July to September 2023. The objective of this research was to determine the optimal growing conditions for <em>Nymphaea minuta</em> based solely on variations in the color temperature of the LED growth lights. This experiment used five light sources to investigate the growth of <em>N. minuta</em> rhizomes, including 1) 6500K-LED growth light (6500K-LED), 2) 4500K-LED growth light (4500K-LED), 3) Mixed 6500K +3500K+450 nm+660 nm+730 nm-LED growth light (Mix LED) and 4) white fluorescent (White-Flu) light sources compared to the control (sunlight). Specimens from each group were planted in a ceramic cup with a 12.7 cm top diameter and a 10.16 cm depth, which was half filled with heavy clay soil. Characterization of the samples was conducted from day 28 to day 84 post-planting to assess growth. Our findings indicate that the responses of <em>N. minuta</em> to different light qualities diverged from those observed in most terrestrial plants. The leaf count under artificial light treatments did not significantly differ from that under sunlight treatment after 84 days. However, the leaf length, leaf width, and petiole length under sunlight treatment were lower than those under artificial light treatments. The Mix LED treatment exhibited the shortest time to flowering (54.75 days), whereas the White-Flu treatment required the longest duration to reach flowering (75.00 days).</p>Apisit ChittawanijRusama MarubodeeNathathai SaeyangManut Aree
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2026-05-202026-05-20310331-03-02 (7 pages)10.22299/apst.2026.273811Mycochemical Composition, Antioxidant Properties, and Cytotoxic Activity of Xylaria papulis Lloyd (Xylariaceae) Mycelia Grown in Static and Shaking Indigenous Liquid Culture Conditions
https://so01.tci-thaijo.org/index.php/APST/article/view/269909
<p>The search for promising and sustainable sources of secondary metabolites with potential biofunctional activities is important for the possible sources of drugs to treat human diseases. Hence, this study qualitatively assessed the mycochemical composition and investigated the biofunctional activities (antioxidant activity and cytotoxic activity) of <em>Xylaria papulis</em> Lloyd mycelia grown in static and shaking indigenous liquid culture medium. Mycochemical screening revealed the presence of several classes of secondary metabolites, such as phenolics, terpenoids, fatty acid derivatives, alkaloids, and sugar derivatives, in mycelial mat (shaking) and in culture spent (static). Mycelial mat (static) and culture spent (shaking) also contain the same classes of secondary metabolites, with the former including essential oils. Mycelial extracts have greater antioxidant property (≤ 43.19±1.63% RSA) and total phenolics (≤ 47.75±2.31 mg/GAE) compared to the culture spent. Culture spent from shaking condition exhibited cytotoxicity (LC50 = 94.39µg/mL) against brine shrimp larvae. These findings indicate that <em>X. papulis</em> (Lloyd) grown in liquid culture medium can be a sustainable source of diverse secondary metabolites with antioxidant properties and cytotoxic activity. Further, cytotoxicity studies against cancer cell lines can also be investigated for future studies.</p>Mark Kevin S. LopezSofronio P. KalawRich Milton R. DulayAngeles M. De LeonRenato G. Reyes
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2026-05-202026-05-20310331-03-03 (10 pages)10.22299/apst.2026.269909Macrofungi Species in Para Rubber Plantation in Kaeng Hang Maeo District, Chanthaburi Province, Thailand
https://so01.tci-thaijo.org/index.php/APST/article/view/280494
<p>This study aimed to investigate the macrofungi species in a para rubber plantation in Kaeng Hang Maeo District, Chanthaburi Province, to identify them by a molecular method, and to evaluate their mode of life, and edibility data. Twenty-three macrofungi samples were collected, of which 16 macrofungi with different fruiting body forms were selected for identification. Based on macro-morphological characteristics, they were divided into 5 groups: (i) <em>Cordyceps</em>, <em>Xylaria</em>, and <em>Daldinia</em>, (ii) Earth tongues, (iii) Gilled fungi, (iv) Polypores and bracket fungi, and (v) Jelly fungi. The gilled fungi (37.5%) were mostly found in this area. Subsequently, the internal transcribed spacer (ITS) was amplified by a polymerase chain reaction (PCR), and its sequence was analyzed. The macrofungi were classified into 2 phyla, 4 classes, 5 orders, 9 families, and 15 genera. Most macrofungi were classified in the phylum Basidiomycota (75%) and the family Polyporaceae (31.25%). However, only 1 sample could not be identified at the species level, which was <em>Gerronema </em>sp. (KM12). Additionally, most of the macrofungi played a role as saprotrophs in the ecosystem (93.75%), and only 1 sample was found to be a pathotroph (6.25%). Although most macrofungi lacked edibility data (87.5%), edible macrofungi, including <em>Schizophyllum commune</em> (KM2) and <em>Dacryopinax spathularia</em> (KM3), were identified (12.5%). However, poisonous macrofungi were not reported in this study.</p>Saowapha SurawutPornpimon KanjanavasChutapa KunsookWinyou PuckdeeSorasak Nak-eiamOnpriya KesornNarumon ApinansawatChawanrat SomnuekPimjai SuwannawongWaritchon Ninlanon
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2026-05-202026-05-20310331-03-05 (13 pages)10.22299/apst.2026.280494Production Performance and Proximate Nutrient and Myco-chemical Composition of Paddy Straw Mushroom, Volvariella volvacea, Isolates Cultivated on Supplemented Banana Leaf Substrates
https://so01.tci-thaijo.org/index.php/APST/article/view/278552
<p><em>Volvariella volvacea</em> (Bull.: Fr.) Singer (Plutaceae), commonly known as the paddy straw mushroom, is widely cultivated in the Philippines, where banana leaves are traditionally used as a substrate. However, standardized protocols for their efficient utilization remain limited. In this study, six isolates of <em>V. volvacea</em> were evaluated, comprising three commercial isolates from San Jose, Nueva Ecija (SJ), Tarlac City (TC), and La Union (LU), and three wild isolates collected from Brgy. Rang-ayan, Muñoz, Nueva Ecija (VVA), Central Luzon State University (VVB), and San Mateo, Rizal (SP). Supplementation of rice bran to banana leaf substrates significantly enhanced fruiting performance in isolates SJ, TC, LU, and SP, yielding biological efficiencies of 9.17%, 17.04%, 7.65%, and 10.98%, respectively. In contrast, coconut water supplementation was more effective for isolates VVA and VVB, with biological efficiencies of 9.22% and 9.61%, respectively, under uniform cultivation conditions. Proximate analysis of the harvested fruiting bodies revealed appreciable amount of carbohydrate (43.8-49.1%), crude protein (23.8-29.7%), crude fiber (10.4-19.7%), ash (12.2-14.2%) and very low crude fat (0.4-0.7%). Phytochemical screening further showed the presence of flavonoids and alkaloids exclusively in isolates SJ and TC. These confirm that both production performance and biochemical composition in <em>V. volvacea</em> are strongly isolate-dependent, highlighting the importance of strain selection and substrate optimization in improving cultivation efficiency and functional properties.</p>Louie S. TapnioRich Milton R. DulayEmelina H. MandiaLlewelyn M. EspirituHarold Ray R. Landingin
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2026-05-212026-05-21310331-03-06 (9 pages)10.22299/apst.2026.278552Cultivation of Ganoderma multipileum on Rubber Wood Sawdust and Coffee Grounds: Growth Performance and Bioactive Properties
https://so01.tci-thaijo.org/index.php/APST/article/view/283370
<p><em>Ganoderma multipileum</em> is a tropical wood-decaying fungus with high capacity for crop waste disposal and promising medicinal properties. In this study, a Vietnamese strain of <em>G. multipileum </em>was identified through morphological characterization and phylogenetic analysis of the internal transcribed spacer (ITS) region. To enhance sustainable mushroom production, two cultivation substrates were compared: rubber tree sawdust by itself (control) and rubber tree sawdust supplemented with 25% coffee grounds. The coffee-supplemented substrate reduced the carbon/nitrogen ratio from 83.87:1 to 41.86:1, accelerated mycelial colonization from 28 to 22 days, and increased the mycelial growth rate from 6.53 to 7.59 mm/day. Ethanolic extracts from fruiting bodies grown on the supplemented substrate showed higher antibacterial activity against both methicillin-sensitive and methicillin-resistant<em> Staphylococcus aureus</em> and <em>Bacillus cereus</em> compared with the control. Antioxidant capacity, as measured by DPPH assay, was reduced in the supplemented treatment, with a higher IC₅₀ value (104.27 μg/mL) compared with the control (47.44 μg/mL). These findings highlight the potential of lignocellulolytic <em>Ganoderma</em> fungi in a circular economy for reducing agro-industrial waste byproducts like sawdust and coffee grounds. An additional benefit accrues because the use of such substrates enhances the cultivation efficiency and bioactive properties of <em>G. multipileum</em>, promoting its application in pharmaceutical and cosmetic production as well as waste reduction.</p>Trang Thi Thu NguyenThanh-Mai Thi NguyenHong Quan NguyenMinh Tu NguyenHoang Duc Nguyen
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2026-05-212026-05-21310331-03-07 (12 pages)10.22299/apst.2026.283370Development of Silk Fibroin/Chitosan Sponge for Wound Dressing
https://so01.tci-thaijo.org/index.php/APST/article/view/279991
<p>Gauze is a traditional wound dressing. However, it often adheres to the wound and is not highly absorbent. To address the limitations of traditional dressings, biopolymers are commonly considered suitable materials for wound-dressing applications. Silk fibroin (SF) is an interesting polymer known for its good water absorption, but it has poor mechanical properties. In this study, chitosan (CS) was selected to improve the mechanical properties of SF. SF/CS sponges were prepared with glycerol as a plasticizer at different ratios of SF to CS: 100/0, 75/25, 50/50, 25/75, and 0/100 (w/w of dry substances) using a freeze-drying process. Their physicochemical properties were investigated, including chemical structure, morphology, mechanical properties, swelling ratio, water uptake, and porosity, as well as cell viability. According to the results, all SF/CS sponges had suitable pore sizes (28–61 µm). The sponges exhibited high water uptake (90–96%) and swelling ratios (900–2,100%). The addition of CS greatly enhanced the mechanical properties of the sponges. Notably, SF/CS sponges at ratios of 50/50, 25/75, and 0/100 showed increases in tensile strength (0.49–0.65 MPa) and elongation at break (71–190%). However, the 0SF/100CS sponge was found to have limitations due to its low porosity (<60%). In addition, the MTT assay confirmed that none of the SF/CS sponges exhibited cytotoxicity. In summary, SF/CS sponges at 50/50 and 25/75 ratios showed potential as biomaterials for wound dressings, offering advantages in water uptake, mechanical properties, swelling ratio, porosity, and non-cytotoxicity.</p>Juthathip PhianphumipongRachasit JeenchamYupaporn RuksakulpiwatPiya-on Numpaisal
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2026-05-212026-05-21310331-03-08 (10 pages)10.22299/apst.2026.279991Utilizing Open-Source GIS and Multi-Criteria Decision Analysis for Onion Cold Storage Site Selection in Nueva Vizcaya, Philippines
https://so01.tci-thaijo.org/index.php/APST/article/view/280902
<p>Onion is an important agricultural commodity in the Philippines, and it provides livelihood to the farmers and contributes to food security in the country. Despite its observed increase in production, challenges remain in terms of postharvest management, particularly due to inadequate storage. This study aimed to locate suitable sites for cold storage infrastructure in the province of Nueva Vizcaya using Geographic Information System (GIS) and Multi-Criteria Decision Analysis (MCDA). Spatial data such as land cover, road network, onion production area, and agricultural markets were used for weighted overlay analysis through Quantum GIS (QGIS). The findings showed that approximately 43.84% (171,997 ha) of the total area of the province is suitable for cold storage of onions, with a large extent of areas in the municipalities of Kayapa, Aritao, Santa Fe, and Bambang. The applicability of the model was demonstrated by the spatial analysis that verified the proposed onion cold storage location in Aritao was in an appropriate location. This exhibited the capability and importance of using an open-source GIS for siting postharvest facilities. It is suggested for future studies to incorporate parameters including socioeconomic indicators, facility capacity, and logistical costs to enhance future investments in agricultural infrastructure.</p>Glenn M. Oca
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2026-05-212026-05-21310331-03-09 (11 pages)10.22299/apst.2026.280902Bio-Based Ion Exchange Resin from Water Hyacinth for Efficient Groundwater Hardness Treatment
https://so01.tci-thaijo.org/index.php/APST/article/view/278761
<p>This study aims to prepare cation exchange resin from the stems and leaves of water hyacinth via the sulfonation process and to evaluate their efficiency and capacity for water softening. The water hyacinth was separated into stems and leaves and was then ground to powder. The powders were subjected to sulfuric acid treatment (sulfonation) to introduce active sulfonic acid (-SO<sub>3</sub>H) groups. Treated materials were classified as stem- and leaf-derived water hyacinth resin. The results revealed that sulfuric acid-treated water hyacinth enhanced the porosity of resin due to the incorporation of sulfonic acid groups. This improvement provided a larger surface area and more active sites for cation exchange compared to untreated samples. Sulfuric acid-treated water hyacinth leaves demonstrated greater hardness removal efficiency compared to stems, attributed to the higher cellulose content in the leaves. The optimal dosage of leaf-derived resin for complete hardness removal (414 mg·L<sup>-1</sup> as CaCO<sub>3</sub>) was determined to be 22.5 g·L<sup>-1</sup>, equivalent to an ion exchange capacity of 0.810 meq·g<sup>-1</sup>. The resin exhibited selective affinity for ions in the order of Mg²⁺ > Ca²⁺ >> Na⁺. Additionally, the resin retained its efficiency after the first and second regeneration cycles using 0.01 M hydrochloric acid, maintaining 100% hardness removal, comparable to the performance of the new resin. These results highlight the potential of bio-based resins derived from water hyacinth leaves as a sustainable, cost-effective solution for water softening. Bio-based resins contribute to environmental conservation, promote eco-friendly materials, and support the circular economy and sustainable resource management principles.</p>Yada PinathaApatsara KrasaesatNichakorn PornnongsanSuwanun JuangjunSaitarn PakakaewPraphaphon ThaochampaWina Rongchapo
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2026-05-222026-05-22310331-03-10 (11 pages)10.22299/apst.2026.278761Protective and Therapeutic Effects of Thai Vegetable Extracts Against Oxidative Stress and Apoptosis in RAW 264.7 Macrophage Cells
https://so01.tci-thaijo.org/index.php/APST/article/view/283574
<p>Consuming vegetables is vital for disease prevention, and Thailand’s diverse vegetable resources are of particular interest. This study investigated the antioxidant and anti-apoptotic activities of ethanolic extracts from 48 Thai vegetable species. Antioxidant capacity was evaluated using FRAP and DPPH assays, and total phenolic content was determined by the Folin-Ciocalteu method. <em>Schinus terebinthifolius</em> (MT) and <em>Caesalpinia mimosoides</em> (KY) exhibited the highest phenolic content and antioxidant activity and were selected for further study. In RAW 264.7 macrophages exposed to H<sub>2</sub>O<sub>2</sub> (200 µM), cell viability decreased to approximately 30%, whereas co-treatment with KY extract (12.5-100 µg/mL) restored viability in a dose-dependent manner, reaching 84.65%, while MT extract increased viability to approximately 50%. Flow cytometric analysis revealed that H<sub>2</sub>O<sub>2</sub> induced apoptosis in ~90% of cells; MT extract reduced apoptotic cells by up to 20.65%, whereas KY extract produced a modest reduction (≤3.37%). RT-qPCR analysis demonstrated the upregulation of antioxidant-related genes (<em>CAT, SOD1</em>) and downregulation of pro-apoptotic genes (<em>p53, caspase-9</em>). Trace-element analysis further suggested that copper, selenium, and zinc may contribute to antioxidant enzyme activity. Collectively, MT and KY extracts exhibit significant antioxidant and anti-apoptotic effects, supporting their potential as non-toxic natural agents for mitigating oxidative stress and apoptosis-related conditions.</p>Adisak PalasapNisachon JangprommaNarintorn RattanataPatcharee BoonsiriSakda DaduangPatcharaporn TippayawatPornsuda MaramingJringjai AreemitPaweena TunakhunJureerut Daduang
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2026-05-222026-05-22310331-03-11 (10 pages)10.22299/apst.2026.283574Accuracy assessment of EnMap-Box machine learning algorithms for land use and land cover classification using Landsat imagery
https://so01.tci-thaijo.org/index.php/APST/article/view/281549
<p>Land use and land cover (LULC) is essential information for multiple users across multidisciplinary sciences. Currently, free downloads of remote sensing data and public software with novel algorithms are available for LULC classification by scientists and researchers. The study’s objectives were (1) to classify and map LULC data for 2022 using random forest (RF) and support vector machine (SVM) classifiers and (2) to assess the thematic accuracy of classified LULC maps with RF and SVM classifiers. The research methodology comprised four significant steps. As a result, the nine LULC classes classified in 2022 using RF and SVM achieve overall accuracy values above 90% and Kappa-hat values above 87%. According to the pairwise Z-test, nine decision tree numbers for RF and 12 combinations of g and C parameters for SVM can classify LULC data for 2022 with insignificant differences in Khat values. In this study, the optimal number of decision trees for LULC classification using RF was 150. Meanwhile, the most suitable parameter for LULC classification by SVM was the Gaussian radial basis function kernel with a g value of 0.01 and a C value of 0.1. However, to apply the EnMap-Box for LULC classification, preparing the training and testing datasets is an important step, and users must collect them carefully. Nevertheless, the research methodology can serve as a guideline for classifying land use and land cover information from Landsat imagery using the EnMap-Box software for urban and land-use planning.</p>Suwit OngsomwangSongkot DasanandaPantip PiyatadsananonSiripon KamontumTanakorn Sritarapipat
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2026-05-252026-05-25310331-03-12 (13 pages)10.22299/apst.2026.281549Strawberry (Fragaria ananassa L.) Fruit Extract as an Antioxidant and Extracellular Matrix Protector for Anti-Photoaging
https://so01.tci-thaijo.org/index.php/APST/article/view/282536
<p>Skin aging, exacerbated by oxidative stress and environmental factors including UV radiation, has led to increased demand for effective anti-aging solutions. Strawberry extract, rich in antioxidants including catechins and flavonoids, has garnered attention for its potential in combating skin aging through its antioxidant and anti-inflammatory properties. This study investigates the anti-aging effects of strawberry (<em>Fragaria ananassa</em> L.) extract, focusing on its antioxidant capacity, inhibition of collagenase and elastase, and its impact on key aging biomarkers. Antioxidant activity was assessed using DPPH and H₂O₂ scavenging assays. The inhibition of collagenase and elastase was evaluated through enzyme activity assays. Molecular docking simulations assessed interactions between bioactive compounds in strawberry extract and anti-aging target proteins, including KEAP1, MMP1, and elastase. Strawberry extract demonstrated marked antioxidant capacity, as reflected by its IC₅₀ values of 229.91 ± 4.42 µg/mL (DPPH) and 234.08 ± 4.20 µg/mL (H₂O₂). It also showed enzyme inhibitory effects, with IC₅₀ values of 112.81 ± 1.96 µg/mL for collagenase and 28.03 ± 0.68 µg/mL for elastase. Cyanidin-3-glucoside showed binding interactions with KEAP1 and elastase (ΔG values of -9.6 and -6.4 kcal/mol), while cyanidanol interacted with MMP1 (ΔG = -9.8 kcal/mol). Strawberry extract demonstrated significant anti-aging and antioxidant effects, both <em>in vitro</em> and through molecular docking simulations, highlighting its potential as an potential anti-aging agent. These results highlight the potential application of strawberry extract in topical skincare products or as a dietary supplement.</p>Wahyu WidowatiHendra SubrotoImelda ImeldaDian PuspitasariYenny NoorVini AyuniReyhan Natadilandes
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2026-05-252026-05-25310331-03-13 (10 pages)10.22299/apst.2026.282536Comparative Effects of Culture Sites on Agar Yield and Quality Parameters of Gracilaria edulis in La Union, Philippines
https://so01.tci-thaijo.org/index.php/APST/article/view/280801
<p>The agar potential of <em>Gracilaria edulis</em> cultured at two coastal sites in La Union, Philippines, was evaluated to determine site-dependent differences in yield and quality. Significant variations (p < 0.05) were observed between Balaoan and Rosario. While Rosario produced greater biomass, Balaoan yielded significantly higher agar. Proximate composition also differed, with Balaoan exhibiting higher protein and carbohydrate contents. Concentrations of inorganic arsenic (i-As), cadmium (Cd), and lead (Pb) were within the permissible limits set by the Department of Environment and Natural Resources for SB-class waters, indicating environmental safety at both sites. Agar quality parameters showed contrasting trends. Rosario samples demonstrated significantly higher viscosity, gel strength, and 3,6-anhydrogalactose content, suggesting superior gel-forming properties. In contrast, Balaoan agar exhibited higher melting and gelling temperatures, as well as higher sulfate levels. These results confirm that culture site significantly influences agar yield and physicochemical properties of <em>G. edulis</em>. The findings provide important insights for optimizing site selection to enhance both productivity and functional quality in commercial agar production.</p>Andie John D. Tadeo
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2026-05-252026-05-25310331-03-14 (9 pages)10.22299/apst.2026.280801Effect of Paraffinic Oil Plasticizer Addition on Kinetics of Rubber Vulcanization Based on Kamal-Sorour and Deng-Isayev Models
https://so01.tci-thaijo.org/index.php/APST/article/view/271422
<p>Plasticizer is an essential component in rubber compounding, characterized by the ability to reduce compound viscosity, improve the dispersion of additives, and significantly affect processing efficiency and vulcanization kinetics. Therefore, this study aimed to investigate the vulcanization kinetics of styrene butadiene rubber (SBR) with varying amounts of paraffinic oil plasticizer. The experimental procedures included mixing rubber and additives, followed by compound testing using a rheometer. The kinetic parameters of the Deng-Isayev and Kamal-Sorour models were determined using the sum of squared error (SSE) optimization of the rheological data. The results showed that increasing the vulcanization temperature and reducing plasticizer content caused a decrease in induction time (<em>t<sub>s2</sub></em>) and optimum vulcanization time (<em>t<sub>c90</sub></em>). Furthermore, the experimental data were accurately modeled using the Kamal-Sorour model. The vulcanization reaction occurred concurrently with the predominance of the autocatalysis mechanism (<em>k<sub>2</sub></em> > <em>k<sub>1</sub></em>), while the values of <em>m</em> and <em>n</em> were inversely proportional to the amount of plasticizer. The vulcanization reaction was primarily influenced by the initial reactant compound rather than the catalytic effect on the product reaction (<em>n</em> > <em>m</em>), showing that high plasticizer content increased energy requirement (<em>E<sub>2</sub></em> > <em>E<sub>1</sub></em>) for autocatalytic reactions.</p>Andri SaputraHersandy Dayu KusumaMertza Fitra AgustianSyaifa Eza SatriaDanuraja Ilmannafia
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2026-05-252026-05-25310331-03-15 (10 pages)10.22299/apst.2026.271422Characteristics of Red Hard Candy with Addition of Roselle (Hibiscus sabdariffa L.) Flower Extracts
https://so01.tci-thaijo.org/index.php/APST/article/view/273126
<p>There is a growing interest in replacing synthetic dyes with natural colouring agents, primarily driven by increasing health concerns among consumers worldwide. This work aimed to reformulate hard candy products through incorporating different levels of roselle flower extracts (6-15 g) as an alternative colouring agent. The samples were also enriched with Javanese long pepper extract as a source of functional components and xylitol as an alternative sweetener. In this work, the properties of hard candy, including pH, solubility time, colour variation (expressed as Red, Green, and Blue – RGB), DPPH radical scavenging (% inhibition), and sensory acceptability, were evaluated. As a result, while pH, colour, and free radical inhibition were significantly altered, the treatments given did not affect solubility time. The pH level decreased consistently as the amount of roselle flower extract increased. Conversely, the RGB values and percentage inhibition increased with greater incorporation of roselle flower extracts into the formulation. In the case of colour, a higher proportion of the extract led to higher RGB values in hard candy, corresponding to a greater level of brightness achieved by F4. Meanwhile, F2 demonstrated the highest red intensity. Furthermore, the treatments resulted in significant effects on all sensory attributes, except aroma. F1 reached the highest sensory score for taste, texture, and overall attributes, while F2 reached the highest for colour. The work results confirm the further use of roselle flower in the formulation of red hard candy.</p>Airiza SekardinaSupriyanto SupriyantoMojiono MojionoHadi Suprapto
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2026-05-252026-05-25310331-03-16 (7 pages)10.22299/apst.2026.273126Physical and textural attributes of rice-sweet potato-watermelon seed extruded snacks: influence of extrusion process conditions
https://so01.tci-thaijo.org/index.php/APST/article/view/278509
<p>The combination of rice flour, sweet potato flour, and watermelon seed powder remains a relatively unexplored resource within the realm of extruded snacks production. Thus, this study investigated the effect of three extrusion process parameters: feed moisture content (FMC, 16-18%), exit barrel temperature (EBT, 120-140 °C), and barrel screw speed (BSS, 300-420 rpm) on rice-sweet potato-watermelon seed extruded snacks. Physical (bulk density, expansion ratio) and textural properties (hardness, fracturability, chewiness, gumminess, and stringiness) were measured using standard procedures. Statistical analysis identified the most influential parameters, with Pareto charts at a 95% confidence level and contour plots visualizing their impacts. Results showed significant variations: bulk density (0.22-0.73 g/cm³), expansion ratio (2.25-2.58), hardness (54.01-464.95 N), fracturability (22.65-176.28 N), chewiness (0.02-5 N), gumminess (0.86-41.84 N), and stringiness (4.83-5.20 mm). EBT had the greatest impact on expansion ratio, while FMC significantly affected hardness, fracturability, chewiness and gumminess, and BSS influenced bulk density and stringiness. The study concluded that extrusion conditions critically shape the physical and textural attributes of the products, providing valuable insights for optimizing extrusion settings to achieve desired textural profiles and enhance overall snack quality.</p>Mayowa S. SanusiAbdulkareem B. BelloHamidu O. SanusiToheeb B. OlaniranEniola D. AdebayoGhali M. YakubHafsat F. BankoleOreoluwa T. AbodunrinIsrael A. Adeniyi
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2026-05-252026-05-25310331-03-17 (11 pages)10.22299/apst.2026.278509Physiological, Yield, and Fruit Quality Responses of Tomato to Irradiated Carrageenan under Drought Stress
https://so01.tci-thaijo.org/index.php/APST/article/view/280990
<p>This study evaluates the effects of Irradiated Carrageenan Biostimulant (ICB) on fruit yield, quality attributes, biomass allocation, and nutrient assimilation in the commercial tomato hybrid <em>Diamante Max F1</em> under varying levels of water stress. The experiment, conducted under greenhouse conditions in Los Baños, Laguna, Philippines, included five treatments: T1 – moderate (partial) drought with ICB foliar spray (PD + S), T2 – moderate (partial) drought without ICB (PD + US), T3 – severe drought with ICB (SD + S), T4 – severe drought without ICB (SD + US), and T5 – a fully irrigated, unsprayed control (WW + US). Results revealed significant main and interaction effects of water deficit and ICB on multiple plant indicators. Among the treatments, T2 (PD + S) resulted in the highest total dry biomass. Nutrient uptake, specifically nitrogen (N), phosphorus (P), and potassium (K), showed marked improvement under T1. Drought stress led to an increased root-to-shoot ratio, indicating adaptive allocation toward root development. Yield traits including fruit count, weight, length, and diameter peaked in the PD + S treatment. Furthermore, ICB application enhanced fruit quality parameters such as total soluble solids and titratable acidity particularly under partial drought. These findings suggest that foliar application of ICB under moderate water stress can improve tomato productivity, nutrient uptake, and fruit quality, indicating its potential as a viable biostimulant for drought-resilient tomato cultivation.</p>Fredelino E. Vecina JrAnnalissa L. AquinoHazel Anne F. Makahiya
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2026-05-252026-05-25310331-03-18 (11 pages)10.22299/apst.2026.280990