Screening of N2-fixing and IAA Producing Bacteria and their Potential Use as Biofertilizer for Rice

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Published: May 18, 2018
DOI: https://doi.org/10.14456/apst.2018.7
Keywords:
Azotobacter Beijerinckia biofertilizer IAA rice rhizosphere

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Pongpat Chumpoo
Graduate Programme, Master’s Degree in Soil Science and Natural Resources Management, Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Arawan Shutsrirung
Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

Abstract

The rice-based farming system is widespread throughout Thailand.  Approximately 98% of the rice is farmed in a paddy field. The long time inefficient use of chemical fertilizers has dramatically degraded the soil quality and polluted the environment. Rhizosphere-associated nitrogen-fixing bacteria are considered one of the possible alternatives to synthetic nitrogen fertilizer for improving rice growth and yield. The objectives of this study were to isolate bacteria from rice rhizosphere and evaluate their potential in N2-fixation and indole 3-acetic acid (IAA) production. A total number of 31 isolates of Azotobacter and 24 isolates of Beijerinckia were obtained from the rice rhizosphere soils. The results showed that Beijerinckia BCk1 performed maximum nitrogenase activity (68.4 nmole 24hr-1 tube-1) and Azotobacter AtMh6 showed the highest IAA production (28.0 mg IAA L-1). Nitrogen uptake and rice seedlings growth promotion by six selected isolates; AtMh6, AtCk1, AtMh1, BCk1, BSt3 and BSt12 with high potential in N2-fixation (64.6, 60.2, 56.6, 68.4, 57.0 and 50.4 nmole 24hr-1 tube-1, respectively), were investigated.  IAA production of the six isolates was 28.0, 7.2, 3.3, 17.3, 1.4 and 6.6 mg IAA L-1, respectively. The application of all the selected isolates provides higher nitrogen uptake and seedlings growth than the uninoculated control.  Among Azotobacter, isolate AtCk1 gave the best seedling growth and 91.7% increase in N uptake over the control.  The Beijerinckia isolate BCk1 exhibited highest nitrogenase activity and also provide the highest nitrogen uptake in rice seedlings (150% over the control). Our results suggested that isolate AtCk1 and BCk1 have a high potential to develop as biofertilizer for rice.

Article Details

How to Cite
Chumpoo, P., & Shutsrirung, A. (2018). Screening of N2-fixing and IAA Producing Bacteria and their Potential Use as Biofertilizer for Rice. Asia-Pacific Journal of Science and Technology, 23(3), APST–23. https://doi.org/10.14456/apst.2018.7
Issue
Vol. 23 No. 3 (2018): JULY-SEP
Section
Research Articles
Author Biography

Arawan Shutsrirung, Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

Assistant Professor Dr. Arawan Shutsrirung
Division of Soil Science
Department of Plant and Soil Science
Faculty of Agriculture
Chiang Mai University, 50200
Chiang Mai THAILAND

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