Effects of polyvinyl alcohol on vitrification of mature and immature bovine oocytes
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Published:
Dec 29, 2017
Keywords:
Bovine oocyte Cryopreservation Ice blocking agent Polyvinyl alcohol Vitrification
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Abstract
The objective of this study was to evaluate the putative effects of low molecular weight (9000-10000 Da) polyvinyl alcohol (PVA) as an ice blocking agent on vitrification of mature (metaphase II stage; MII) and immature (germinal vesicle stage; GV) bovine oocytes. In experiment 1, the effect of two concentrations of PVA (0.1% and 1%) on the viability of vitrified-warmed MII oocytes was determined using fluorescein diacetate staining. The results showed that the viability of vitrified-warmed MII oocytes was not significantly different among the various groups (vitrified control; 65.6%, +0.1 %PVA; 83.3%, and +1.0 %PVA; 73.7%). In experiment 2, the effect of 0.1% PVA on fertilization and developmental competence of vitrified-warmed MII oocytes was determined. The blastocyst development of oocytes in 0.1%PVA group was significantly higher (p£0.05) than that of the vitrified control group (10.1% and 3%, respectively), but significantly lower (p£0.05) than that of the fresh control group (59.1%). In experiment 3, the maturation rate of vitrified-warmed GV oocytes was determined. The percentages of vitrified-warmed GV oocytes reaching the MII stage were not different among three maturation periods (22h IVM; 33.3%, 24 h IVM; 54.5%, and 26 h IVM; 31.3%). In experiment 4, the effect of 0.1% PVA on fertilization and developmental competence of vitrified-warmed GV oocytes was determined. The proportions of blastocysts were not different between the vitrified control and 0.1% PVA groups (1.5% and 0.8%, respectively). In conclusion, the results showed that a low molecular weight PVA at a concentration of 0.1% improved the vitrification of mature bovine oocytes, but lacked a positive effect with immature oocytes.
Article Details
How to Cite
Chumnandee, C., Sirimungkararat, S., Saksirirat, W., & Srijankam, K. (2017). Effects of polyvinyl alcohol on vitrification of mature and immature bovine oocytes. Asia-Pacific Journal of Science and Technology, 22(4), APST–22. https://doi.org/10.14456/apst.2017.33
Section
Research Articles
References
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[18] Fasano, G., Vannin, A.S., Biramane, J., Delbaere, A., Englert, Y., 2010. Cryopreservation of human failed maturation oocytes shows that vitrification gives superior outcomes to slow cooling. Cryobiology 61, 243-247.
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[23] Wowk, B., Leitl, E., Rasch, C.M., Mesbah-Karimi, N., Harris, S.B., Fahy, G.M., 2000. Vitrification enhancement by synthetic ice blocking agents. Cryobiology 40, 228-236.
[24] O’Neil, L., Paynter, S.J., Fuller, B.J., Shaw, R.W., DeVries, A.L., 1998. Vitrification of mature mouse oocytes in a 6 M Me2SO solution supplemented with antifreeze glycoproteins: the effect of temperature. Cryobiology 37, 59-66.
[25] Naitana, S., Ledda, S., Loi, P., Leoni, G., Bogliolo, L., Dattena, M., Cappai, P., 1997. Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. Animal Reproduction Science 48, 247–256.
[26] Asada, M., Ishibashi, S., Ikumi, S., Fukui, Y., 2002. Effect of polyvinyl alcohol (PVA) concentration during vitrification of in vitro mature bovine oocytes. Theriogenology 58, 1199-1208.
[27] Checura, C.M., Seidel, G.E., Jr., 2007. Effect of macromolecules in solutions for vitrification of mature bovine oocytes. Theriogenology 67, 919-930.
[28] Kong, I.K., Lee, S.I., Cho, S.G., Cho, S.K., Park, C.S., 2000. Comparison of open pulled straw (OPS) VS glass micropipette (GMP) vitrification in mouse blastocyst. Theriogenology 53, 1817-1826.
[29] Papis, K., Shimizu, M., Izaike, Y., 2000. Factors affecting the survivability of bovine oocytes vitrified in droplets. Theriogenology 54, 651-58.
[30] Shi, W.Q., Zhu, S.E., Zhang, D., Wang, W.H., Tang, G.L., Hou, Y.P., Tian, S.J., 2006. Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes. Reproduction 131, 795-804.
[31] Morató Roser, Izquierdo Dolors, Albarracín José Luis, Anguita Begoña, Palomo María Jesús, Jiménez‐Macedo Ana Raquel, Paramio María Teresa, Mogas Teresa, 2007. Effects of pre‐treating in vitro‐matured bovine oocytes with the cytoskeleton stabilizing agent taxol prior to vitrification. Molecular Reproduction and Development 75, 191–201.
[32] Kim, D.-H., Park, H.-S., Kim, S.-W., Hwang, I.-S., Yang, B.-C., Im, G.-S., Chung, H.-J., Seong, H.-W., Moon, S.-J., Yang, B.-S., 2007. Vitrification of Immature Bovine Oocytes by the Microdrop Method. Journal of Reproduction and Development 53, 843–851.
[33] Green, V.S., Stott, D.E., Diack, M., 2006. Assay for fluorescein diacetate hydrolytic activity: optimization for soil samples. Soil Biology and Biochemistry 38, 693-701.
[34] Zhou, X.L., Naib, A.A., Sun, D.W., Lonergan, P., 2010. Bovine oocyte vitrification using the cryotop method: effect of cumulus cells and vitrification protocol on survival and subsequent development. Cryobiology 61, 66-72.
[35] Yang, B.-C., Im, G.-S., Chang, W.-K., Lee, Y.-K., Oh, S.-J., Jin, D.-I., Im, K.-S., Lee, C.-K., 2003. Survival and In Vitro Development of Immature Bovine Oocytes Cryopreserved by Vitrification, Survival and In Vitro Development of Immature Bovine Oocytes Cryopreserved by Vitrification. Asian-Australasian Journal of Animal Sciences, Asian-Australasian Journal of Animal Sciences 16, 23–28.
[36] Sripunya, N., Liang, Y., Panyawai, K., Srirattana, K., Ngernsoungnern, A., Ngernsoungnern, P., Ketudat-Cairns, M., Parnpai, R., 2014. Cytochalasin B efficiency in the cryopreservation of immature bovine oocytes by Cryotop and solid surface vitrification methods. Cryobiology 69, 496–499.
[37] Schmidt, M., Hyttel, P., Avery, B., Greve, T., 1995. Ultrastructure of in vitro matured bovine oocytes after controlled freezing in 10% glycerol. Animal Reproduction Science 37, 281-90.
[38] Dai, J., Wu, C., Muneri, C.W., Niu, Y., Zhang, S., Rui, R., Zhang, D., 2015. Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability. Cryobiology 71, 291–298.
[39] Stachowiak, E.M., Papis, K., Kruszewski, M., Iwanenko, T., Bartomiejezyk, T., Modlinski, J.A., 2009. Comparison of the levels of DNA damage using Comet assay in bovine oocytes subjected to selected vitrification methods. Reproduction in Domestic Animals 44, 653-8.
[40] Wowk, B., 2002. Polyvinyl alcohol compounds for inhibition of ice growth. US6391224B1.
[41] Deller, R.C., Vatish, M., Mitchell, D.A., Gibson, M.I., 2014. Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing. Nature Communications 5, 3244.
[42] Faheem, M.S., Baron, E., Carvalhais, I., Chaveiro, A., Pavani, K., da Silva, F.M., 2014. The effect of vitrification of immature bovine oocytes to the subsequent in vitro development and gene expression. Zygote 26, 1-10.
[43] Gilchrist, R.B., Lane, M., Thompson, J.G., 2008. Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality. Human Reproduction Update 14, 159–177.
[2] Dinnyes, A., Dai, Y., Jiang, S., Yang, X., 2000. High developmental rates of vitrified bovine oocytes following parthenogenetic activation, in vitro fertilization, and somatic cell nuclear transfer. Biology of Reproduction 63, 513-518.
[3] Atabay, E.C., Takahashi, Y., Katagiri, S., Nagano, M., Koga, A., Kanai, Y., 2004. Vitrification of bovine oocytes and its application to intergeneric somatic cell nucleus transfer. Theriogenology 61, 15-23.
[4] Gosden, R.G., 2005. Prospects for oocyte banking and in vitro maturation. Journal of the National Cancer Institute. Monographs 34, 60-3.
[5] Mara, L., Casu, S., Carta, A., Dettena, M., 2013. Cryobanking of farm animal gametes and embryos as a means of conserving livestock genetics. Animal Reproduction Science 138, 25-38.
[6] Grifo, J.A., Noyes, N., 2009. Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preservation for female cancer patients. Fertility and Sterility 93, 391-396.
[7] Fuku, E., Xia, L., Downey, B.R., 1995. Ultrastructure changes in bovine oocytes cryopreserved by vitrification. Cryobiology 32, 139-56.
[8] Saragusty, J., Arav, A., 2011. Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction 141, 1-19.
[9] Vieira, A.D., Forell, F., Feltrin, C., Rodrigues, J.L., 2008. Calves born after direct transfer of vitrified bovine in vitro-produced blastocysts derived from vitrified immature oocytes. Reproduction in Domestic Animals 43, 314–318.
[10] Somfai, T., Yoshioka, K., Tanihara, F., Kaneko, H., Noguchi, J., Naomi, K., Nagai, T., Kikuchi, K., 2014. Generation of Live Piglets from Cryopreserved Oocytes for the First Time Using a Defined System for In Vitro Embryo Production. PLoS ONE 9, e97731.
[11] Kohaya, N., Fujiwara, K., Ito, J., Kashiwazaki, N., 2013. Generation of Live Offspring from Vitrified Mouse Oocytes of C57BL/6J Strain. PLoS ONE 8, e58063.
[12] Kim, T.J., Hong, S.W., 2011. Successful live birth from vitrified oocytes after 5 years of cryopreservation. Journal of Assisted Reproduction and Genetics 28, 73-6.
[13] Massip, A., 2003. Cryopreservation of bovine oocytes: current status and recent developments. Reproduction Nutrition Development 43, 325-330.
[14] Morato, R., Mogas, T., Hyttel, P.M., 2008a. Ultrastructure of bovine oocytes exposed to Taxol prior to OPS vitrification. Molecular Reproduction and Development 75, 1318-26.
[15] Diez, C., Duque, P., Gomez, E., Hidalgo, C.O., Tamargo, C., Rodriguez, A., 2005. Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability. Theriogenology 64, 317-333.
[16] Rall, W.F., Fahy, G.M., 1985. Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification. Nature 313, 573-557.
[17] Cao, Y.-X., Xing, Q., Li, L., Cong, L., Zhang, Z.-G., Wei, Z.-L., Zhou, P., 2009. Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification. Fertility and Sterility 92, 1306–1311.
[18] Fasano, G., Vannin, A.S., Biramane, J., Delbaere, A., Englert, Y., 2010. Cryopreservation of human failed maturation oocytes shows that vitrification gives superior outcomes to slow cooling. Cryobiology 61, 243-247.
[19] Sprícigo, J.F.W., Morais, K., Ferreira, A.R., Machado, G.M., Gomes, A.C.M., Rumpf, R., Franco, M.M., Dode, M. a. N., 2014. Vitrification of bovine oocytes at different meiotic stages using the Cryotop method: assessment of morphological, molecular and functional patterns. Cryobiology 69, 256–265.
[20] Ezoe, K., Yabuuchi, A., Tani, T., Mori, C., Miki, T., Takayama, Y., Beyhan, Z., Kato, Y., Okuno, T., Kubayashi, T., Kato, K., 2015. Developmental Competence of Vitrified-Warmed Bovine Oocytes at the Germinal-Vesicle Stage is Improved by Cyclic Adenosine Monophosphate Modulators during In Vitro Maturation. PLoS ONE 10, e0126801.
[21] Gupta, M.K., Uhm, S.J., Lee, H.T., 2007. Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification. Theriogenology 67, 238-248.
[22] Aono, A., Nagatomo, H., Takuma, T., Nonaka, R., Ono, Y., Wada, Y., Abe, Y., Takahashi, M., Watanabe, T., Kawahara, M., 2013. Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle. Theriogenology 79, 1146–1152.e1.
[23] Wowk, B., Leitl, E., Rasch, C.M., Mesbah-Karimi, N., Harris, S.B., Fahy, G.M., 2000. Vitrification enhancement by synthetic ice blocking agents. Cryobiology 40, 228-236.
[24] O’Neil, L., Paynter, S.J., Fuller, B.J., Shaw, R.W., DeVries, A.L., 1998. Vitrification of mature mouse oocytes in a 6 M Me2SO solution supplemented with antifreeze glycoproteins: the effect of temperature. Cryobiology 37, 59-66.
[25] Naitana, S., Ledda, S., Loi, P., Leoni, G., Bogliolo, L., Dattena, M., Cappai, P., 1997. Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. Animal Reproduction Science 48, 247–256.
[26] Asada, M., Ishibashi, S., Ikumi, S., Fukui, Y., 2002. Effect of polyvinyl alcohol (PVA) concentration during vitrification of in vitro mature bovine oocytes. Theriogenology 58, 1199-1208.
[27] Checura, C.M., Seidel, G.E., Jr., 2007. Effect of macromolecules in solutions for vitrification of mature bovine oocytes. Theriogenology 67, 919-930.
[28] Kong, I.K., Lee, S.I., Cho, S.G., Cho, S.K., Park, C.S., 2000. Comparison of open pulled straw (OPS) VS glass micropipette (GMP) vitrification in mouse blastocyst. Theriogenology 53, 1817-1826.
[29] Papis, K., Shimizu, M., Izaike, Y., 2000. Factors affecting the survivability of bovine oocytes vitrified in droplets. Theriogenology 54, 651-58.
[30] Shi, W.Q., Zhu, S.E., Zhang, D., Wang, W.H., Tang, G.L., Hou, Y.P., Tian, S.J., 2006. Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes. Reproduction 131, 795-804.
[31] Morató Roser, Izquierdo Dolors, Albarracín José Luis, Anguita Begoña, Palomo María Jesús, Jiménez‐Macedo Ana Raquel, Paramio María Teresa, Mogas Teresa, 2007. Effects of pre‐treating in vitro‐matured bovine oocytes with the cytoskeleton stabilizing agent taxol prior to vitrification. Molecular Reproduction and Development 75, 191–201.
[32] Kim, D.-H., Park, H.-S., Kim, S.-W., Hwang, I.-S., Yang, B.-C., Im, G.-S., Chung, H.-J., Seong, H.-W., Moon, S.-J., Yang, B.-S., 2007. Vitrification of Immature Bovine Oocytes by the Microdrop Method. Journal of Reproduction and Development 53, 843–851.
[33] Green, V.S., Stott, D.E., Diack, M., 2006. Assay for fluorescein diacetate hydrolytic activity: optimization for soil samples. Soil Biology and Biochemistry 38, 693-701.
[34] Zhou, X.L., Naib, A.A., Sun, D.W., Lonergan, P., 2010. Bovine oocyte vitrification using the cryotop method: effect of cumulus cells and vitrification protocol on survival and subsequent development. Cryobiology 61, 66-72.
[35] Yang, B.-C., Im, G.-S., Chang, W.-K., Lee, Y.-K., Oh, S.-J., Jin, D.-I., Im, K.-S., Lee, C.-K., 2003. Survival and In Vitro Development of Immature Bovine Oocytes Cryopreserved by Vitrification, Survival and In Vitro Development of Immature Bovine Oocytes Cryopreserved by Vitrification. Asian-Australasian Journal of Animal Sciences, Asian-Australasian Journal of Animal Sciences 16, 23–28.
[36] Sripunya, N., Liang, Y., Panyawai, K., Srirattana, K., Ngernsoungnern, A., Ngernsoungnern, P., Ketudat-Cairns, M., Parnpai, R., 2014. Cytochalasin B efficiency in the cryopreservation of immature bovine oocytes by Cryotop and solid surface vitrification methods. Cryobiology 69, 496–499.
[37] Schmidt, M., Hyttel, P., Avery, B., Greve, T., 1995. Ultrastructure of in vitro matured bovine oocytes after controlled freezing in 10% glycerol. Animal Reproduction Science 37, 281-90.
[38] Dai, J., Wu, C., Muneri, C.W., Niu, Y., Zhang, S., Rui, R., Zhang, D., 2015. Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability. Cryobiology 71, 291–298.
[39] Stachowiak, E.M., Papis, K., Kruszewski, M., Iwanenko, T., Bartomiejezyk, T., Modlinski, J.A., 2009. Comparison of the levels of DNA damage using Comet assay in bovine oocytes subjected to selected vitrification methods. Reproduction in Domestic Animals 44, 653-8.
[40] Wowk, B., 2002. Polyvinyl alcohol compounds for inhibition of ice growth. US6391224B1.
[41] Deller, R.C., Vatish, M., Mitchell, D.A., Gibson, M.I., 2014. Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing. Nature Communications 5, 3244.
[42] Faheem, M.S., Baron, E., Carvalhais, I., Chaveiro, A., Pavani, K., da Silva, F.M., 2014. The effect of vitrification of immature bovine oocytes to the subsequent in vitro development and gene expression. Zygote 26, 1-10.
[43] Gilchrist, R.B., Lane, M., Thompson, J.G., 2008. Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality. Human Reproduction Update 14, 159–177.