Effect of dietary Bacillus pumilus A1_YM_1 on growth, intestinal morphology and some hematological parameters of hybrid catfish (Clarias macrocephalus × Clarias gariepinus)
Article Sidebar
Published:
Feb 1, 2019
Keywords:
Bacillus pumilus Hybrid catfish Growth Intestinal morphology Hematology
Main Article Content
Abstract
A 60 day feeding trial was performed to examine the dietary supplementation of Bacillus pumilus A1_YM_1 (BP) on growth, intestinal morphology and some hematological parameters of hybrid catfish (Clarias macrocephalus × Clarias gariepinus). Fish with an average weight of 8.55±0.18 g were divided into 4 treatments with 3 replications. The control treatment was fed with the basal diets, whereas the experimental treatments were fed the basal diet incorporated with 1×106, 1×107 and 1×108 CFU/g. The results indicated that fish fed the diets supplemented with BP significantly enhanced final weight, weight gain, specific growth rate, average daily gain and feed conversion efficiency when compared to the control diet (P<0.05). There were no significant differences in survival rate and condition factor between the experimental and control groups (P>0.05). Hematological evaluation showed supplementation of BP in the fish diets significantly increased red blood cell, white blood cell, lymphocyte and neutrophil when compared to the control diet (P<0.05). Histological study of different parts (anterior, middle and posterior) of intestine proper revealed that dietary supplementation of BP significantly enhanced intestinal villi width, intestinal villi height, thicknesses of muscular layers, goblet cell number and microvillus height when compared to the control (P<0.05). General feed intake behavior and health as well as feed acceptability of the treatments were the same as the control. It was suggested that the optimum dietary level of BP for catfish cultivation observed was 1×107 CFU/g. Thus, the results of this study support the use of BP in diets as a probiotic in order to enhance growth, intestinal morphology and hematology of catfish.
Article Details
How to Cite
Munglue, P., Kronghinrach, K., Rattana, K., Sangchanjiradet, S., & Dasri, K. (2019). Effect of dietary Bacillus pumilus A1_YM_1 on growth, intestinal morphology and some hematological parameters of hybrid catfish (Clarias macrocephalus × Clarias gariepinus). Asia-Pacific Journal of Science and Technology, 24(2), APST–24. https://doi.org/10.14456/apst.2019.13
Section
Research Articles
References
[1] Senanan W, Kapuscinski AR, Na-Nakorn U, Miller LM. Genetic impacts of hybrid catfish farming (Clarias macrocephalus × C. gariepinus) on native catfish populations in central Thailand. Aquaculture. 2004;235(1-4):167-84.
[2] Biswas G, Korenaga H, Nagamine R, Takayama H, Kawahara S, Takeda S, Kikuchi Y, Dashnyam B, Kono T, Sakai M. Cytokine responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells induced with heat-killed probiotics isolated from the Mongolian dairy products. Fish Shellfish Immunol. 2013;34(5):1170-177.
[3] Nayak SK. Probiotics and immunity: a fish perspective. Fish Shellfish Immunol. 2010;29(1):2-14.
[4] Welker TL, Lim C. Use of probiotics in diets of tilapia. J Aquac Res Development. 2011;S1:014.
[5] Cruz PM, Ibáñez AL, Monroy Hermosillo OA, Ramírez Saad HC. Use of probiotics in aquaculture. ISRN Microbiol. 2012;1-14.
[6] Truong Thy HT, Tri NN, Quy OM, Fotedar R, Kannika K, Unajak S, Areechon N. Effects of the dietary supplementation of mixed probiotic spores of Bacillus amyloliquefaciens 54A, and Bacillus pumilus 47B on growth, innate immunity and stress responses of striped catfish (Pangasianodon hypophthalmus). Fish Shellfish Immunol. 2017;60:391-99.
[7] Afrilasari W, Widanarni, Meryandini A. Effect of probiotic Bacillus megaterium PTB 1.4 on the population of intestinal microflora, digestive enzyme activity and the growth of catfish (Clarias sp.). HAYATI J Biosci. 2016;23(4):168-72.
[8] Ghosh K, Sen SK, Ray AK. Characterization of Bacilli isolated from the gut of Rohu, Labeo rohita, fingerlings and its significance in digestion. J Appl Aquaculture. 2002;12(3).33-42.
[9] Aly SM, Mohamed MF, John G. Effect of probiotics on the survival, growth and challenge infection in Tilapia nilotica (Oreochromis niloticus). Aquac Res. 2008;39(6):647-56.
[10] Aly SM, Abd-El-Rahman AM, John G, Mohamed MF. Characterization of some bacteria isolated from Oreochromis niloticus and their potential use as probiotics. Aquaculture. 2008;277(1-2):1-6.
[11] Dasri K, Prawitthana S, Sangchanjiradet S. Indole-3acetic acid production and antagonistic inhibition of Alternaria sp. by Bacillus pumilus isolated from biofertilizer. Proceedings of the 10th National Kasetsart University Kamphaeng Saen Conference; 2003 Dec 6-7; Nakhon Prathom, Thailand; 2003.
[12] Adeoye AA, Yomla R, Jaramillo-Torres A, Rodiles A, Merrifield DL, Davies SJ. Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome. Aquaculture. 2016;463:61-70.
[13] Shabanzadeh S, Shapoori M, Sheikhzadeh N, Nofouzi K, Oushani AK, Enferadi MH, Mardani K, Shahbazfar AA. Growth performance, intestinal histology, and biochemical parameters of rainbow trout (Oncorhynchus mykiss) in response to dietary inclusion of heat-killed Gordonia bronchialis. Fish Physiol Biochem. 2016;42(1):65-71.
[14] Cerezuela R, Fumanal M, Tapia-Paniagua ST, Meseguer J, Moriñigo ΆM, Esteban MA. Histological alterations and microbial ecology of the intestine in gilthead sea bream (Sparus aurata L.) fed dietary probiotics and microalgae. Cell Tissue Res. 2012;350(3):477-89.
[15] Han B, Long WQ, He JY, Liu YJ, Si YQ, Tian LX. Effects of dietary Bacillus licheniformis on growth performance, immunological parameters, intestinal morphology and resistance of juvenile Nile tilapia (Oreochromis niloticus) to challenge infections. Fish Shellfish Immunol. 2015;46(2):225-31.
[16] Fang C, Ma M, Ji H, Ren T, Mims SD. Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula, fed dietary probiotics. Fish Physiol Biochem. 2015;41(1):91-105.
[17] Munglue P. Effects of lotus (Nelumbo nucifera Gaertn.) stamen extract on growth performance, feed utilization and intestinal morphology of catfish (Clarias gariepinus). KKU Res J. 2016;21(2):7-17.
[18] Firouzbakhsh F, Noori F, Khalesi MK, Jani-Khalili K. Effects of a probiotic, protexin, on the growth performance and hematological parameters in the Oscar (Astronotus ocellatus) fingerlings. Fish Physiol Biochem. 2011;37(4):833-42.
[19] Zhu H, Liu H, Yan J, Wang R, Liu L. Effect of yeast polysaccharide on some hematologic parameter and gut morphology in channel catfish (Ictalurus punctatus). Fish Physiol Biochem. 2012;38(5):1441-447.
[20] Balcázar JL, Decamp O, Vendrell D, de Blas I, Ruiz-Zarzuela I. Health and nutritional properties of probiotics in fish and shellfish. Microb Ecol Health Dis. 2006;18(2):65-70.
[21] Reda RM, Selim KM. Evaluation of Bacillus amyloliquefaciens on the growth performance, intestinal morphology, hematology and body composition of Nile tilapia, Oreochromis niloticus. Aquacult Int. 2015;23(1):203-17.
[22] Pirarat N, Pinpimai K, Endo M, Katagiri T, Ponpornpisit A, Chansue N, Maita M. Modulation of intestinal morphology and immunity in nile tilapia (Oreochromis niloticus) by Lactobacillus rhamnosus GG. Res Vet Sci. 2011;91(3):e92-e97.
[23] Blottière HM, Buecher B, Galmiche JP, Cherbut C. Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation. Proc Nutr Soc. 2003;62(1):101-06.
[24] EL-Haroun ER, Goda AMA-S, Kabir Chowdhury A. Effect of dietary probiotic Biogen® supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L.). 2006;37(14):1473-480.
[25] Silva TFA, Petrillo TR, Yunis-Aguinaga J, Marcusso PF, da Silva Claudiano G, de Moraes FR, de Moraes JRE. Effects of the probiotic Bacillus amyloliquefaciens on growth performance, hematology and intestinal morphometry in cage-reared Nile tilapia. Lat Am J Aquat Res. 2015;43(5):963-71.
[26] Harper GM, Monfort M, Saoud IP, Emery MJ, Mustafa S, Rawling MD, Eynon B, Davies SJ, Merrifield DL. An ex vivo approach to studying the interactions of Pediococcus acidilactici and Vibrio (Listonella) anguillarum in the anterior intestine of rainbow trout Oncorhynchus mykiss. J Aquac Res Development. 2011;S1:004.
[27] Merrifield DL, Harper GM, Dimitroglou A, Ringø A, Davies SJ. Possible influence of probiotic adhesion to intestinal mucosa on the activity and morphology of rainbow trout (Oncorhynchus mykiss) enterocytes. Aquac Res. 2010;41(8):1268-272.
[28] Matur E, Eraslan E. The impact of probiotics on the gastrointestinal physiology. In: New advances in the basic and clinical gastroenterology. Brzozowski, T. (Ed.). INTECH Open Access Publisher; 2012.
[29] Dahiya T, Sihag RC, Gahlawat SK. Effect of probiotics on the haematological parameters of indian magur (Clarius batrachus L.). J Fish Aquat Sci. 2012;7(4):279-90.
[30] Hemaiswarya S, Raja R, Ravikumar R, Carvalho IS. Mechanism of action of probiotics. Braz Arch Biol Technol. 2013;56(1):113-9.
[31] Garcia-marengoni N, De Moura MC, Tavares N, De Oliveira E. Use of probiotics Bacillus cereus var. toyoi and Bacillus subtilis C-3102 in the diet of juvenile Nile tilapia cultured in cages. Lat Am J Aquat Res. 2015;43(3):601-6.
[2] Biswas G, Korenaga H, Nagamine R, Takayama H, Kawahara S, Takeda S, Kikuchi Y, Dashnyam B, Kono T, Sakai M. Cytokine responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells induced with heat-killed probiotics isolated from the Mongolian dairy products. Fish Shellfish Immunol. 2013;34(5):1170-177.
[3] Nayak SK. Probiotics and immunity: a fish perspective. Fish Shellfish Immunol. 2010;29(1):2-14.
[4] Welker TL, Lim C. Use of probiotics in diets of tilapia. J Aquac Res Development. 2011;S1:014.
[5] Cruz PM, Ibáñez AL, Monroy Hermosillo OA, Ramírez Saad HC. Use of probiotics in aquaculture. ISRN Microbiol. 2012;1-14.
[6] Truong Thy HT, Tri NN, Quy OM, Fotedar R, Kannika K, Unajak S, Areechon N. Effects of the dietary supplementation of mixed probiotic spores of Bacillus amyloliquefaciens 54A, and Bacillus pumilus 47B on growth, innate immunity and stress responses of striped catfish (Pangasianodon hypophthalmus). Fish Shellfish Immunol. 2017;60:391-99.
[7] Afrilasari W, Widanarni, Meryandini A. Effect of probiotic Bacillus megaterium PTB 1.4 on the population of intestinal microflora, digestive enzyme activity and the growth of catfish (Clarias sp.). HAYATI J Biosci. 2016;23(4):168-72.
[8] Ghosh K, Sen SK, Ray AK. Characterization of Bacilli isolated from the gut of Rohu, Labeo rohita, fingerlings and its significance in digestion. J Appl Aquaculture. 2002;12(3).33-42.
[9] Aly SM, Mohamed MF, John G. Effect of probiotics on the survival, growth and challenge infection in Tilapia nilotica (Oreochromis niloticus). Aquac Res. 2008;39(6):647-56.
[10] Aly SM, Abd-El-Rahman AM, John G, Mohamed MF. Characterization of some bacteria isolated from Oreochromis niloticus and their potential use as probiotics. Aquaculture. 2008;277(1-2):1-6.
[11] Dasri K, Prawitthana S, Sangchanjiradet S. Indole-3acetic acid production and antagonistic inhibition of Alternaria sp. by Bacillus pumilus isolated from biofertilizer. Proceedings of the 10th National Kasetsart University Kamphaeng Saen Conference; 2003 Dec 6-7; Nakhon Prathom, Thailand; 2003.
[12] Adeoye AA, Yomla R, Jaramillo-Torres A, Rodiles A, Merrifield DL, Davies SJ. Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome. Aquaculture. 2016;463:61-70.
[13] Shabanzadeh S, Shapoori M, Sheikhzadeh N, Nofouzi K, Oushani AK, Enferadi MH, Mardani K, Shahbazfar AA. Growth performance, intestinal histology, and biochemical parameters of rainbow trout (Oncorhynchus mykiss) in response to dietary inclusion of heat-killed Gordonia bronchialis. Fish Physiol Biochem. 2016;42(1):65-71.
[14] Cerezuela R, Fumanal M, Tapia-Paniagua ST, Meseguer J, Moriñigo ΆM, Esteban MA. Histological alterations and microbial ecology of the intestine in gilthead sea bream (Sparus aurata L.) fed dietary probiotics and microalgae. Cell Tissue Res. 2012;350(3):477-89.
[15] Han B, Long WQ, He JY, Liu YJ, Si YQ, Tian LX. Effects of dietary Bacillus licheniformis on growth performance, immunological parameters, intestinal morphology and resistance of juvenile Nile tilapia (Oreochromis niloticus) to challenge infections. Fish Shellfish Immunol. 2015;46(2):225-31.
[16] Fang C, Ma M, Ji H, Ren T, Mims SD. Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula, fed dietary probiotics. Fish Physiol Biochem. 2015;41(1):91-105.
[17] Munglue P. Effects of lotus (Nelumbo nucifera Gaertn.) stamen extract on growth performance, feed utilization and intestinal morphology of catfish (Clarias gariepinus). KKU Res J. 2016;21(2):7-17.
[18] Firouzbakhsh F, Noori F, Khalesi MK, Jani-Khalili K. Effects of a probiotic, protexin, on the growth performance and hematological parameters in the Oscar (Astronotus ocellatus) fingerlings. Fish Physiol Biochem. 2011;37(4):833-42.
[19] Zhu H, Liu H, Yan J, Wang R, Liu L. Effect of yeast polysaccharide on some hematologic parameter and gut morphology in channel catfish (Ictalurus punctatus). Fish Physiol Biochem. 2012;38(5):1441-447.
[20] Balcázar JL, Decamp O, Vendrell D, de Blas I, Ruiz-Zarzuela I. Health and nutritional properties of probiotics in fish and shellfish. Microb Ecol Health Dis. 2006;18(2):65-70.
[21] Reda RM, Selim KM. Evaluation of Bacillus amyloliquefaciens on the growth performance, intestinal morphology, hematology and body composition of Nile tilapia, Oreochromis niloticus. Aquacult Int. 2015;23(1):203-17.
[22] Pirarat N, Pinpimai K, Endo M, Katagiri T, Ponpornpisit A, Chansue N, Maita M. Modulation of intestinal morphology and immunity in nile tilapia (Oreochromis niloticus) by Lactobacillus rhamnosus GG. Res Vet Sci. 2011;91(3):e92-e97.
[23] Blottière HM, Buecher B, Galmiche JP, Cherbut C. Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation. Proc Nutr Soc. 2003;62(1):101-06.
[24] EL-Haroun ER, Goda AMA-S, Kabir Chowdhury A. Effect of dietary probiotic Biogen® supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L.). 2006;37(14):1473-480.
[25] Silva TFA, Petrillo TR, Yunis-Aguinaga J, Marcusso PF, da Silva Claudiano G, de Moraes FR, de Moraes JRE. Effects of the probiotic Bacillus amyloliquefaciens on growth performance, hematology and intestinal morphometry in cage-reared Nile tilapia. Lat Am J Aquat Res. 2015;43(5):963-71.
[26] Harper GM, Monfort M, Saoud IP, Emery MJ, Mustafa S, Rawling MD, Eynon B, Davies SJ, Merrifield DL. An ex vivo approach to studying the interactions of Pediococcus acidilactici and Vibrio (Listonella) anguillarum in the anterior intestine of rainbow trout Oncorhynchus mykiss. J Aquac Res Development. 2011;S1:004.
[27] Merrifield DL, Harper GM, Dimitroglou A, Ringø A, Davies SJ. Possible influence of probiotic adhesion to intestinal mucosa on the activity and morphology of rainbow trout (Oncorhynchus mykiss) enterocytes. Aquac Res. 2010;41(8):1268-272.
[28] Matur E, Eraslan E. The impact of probiotics on the gastrointestinal physiology. In: New advances in the basic and clinical gastroenterology. Brzozowski, T. (Ed.). INTECH Open Access Publisher; 2012.
[29] Dahiya T, Sihag RC, Gahlawat SK. Effect of probiotics on the haematological parameters of indian magur (Clarius batrachus L.). J Fish Aquat Sci. 2012;7(4):279-90.
[30] Hemaiswarya S, Raja R, Ravikumar R, Carvalho IS. Mechanism of action of probiotics. Braz Arch Biol Technol. 2013;56(1):113-9.
[31] Garcia-marengoni N, De Moura MC, Tavares N, De Oliveira E. Use of probiotics Bacillus cereus var. toyoi and Bacillus subtilis C-3102 in the diet of juvenile Nile tilapia cultured in cages. Lat Am J Aquat Res. 2015;43(3):601-6.