The effects of vitrification method on the seed survival and genetic stability of Rhynchostylis gigantea (Lindl.) Ridl

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Published: Sep 18, 2020
DOI: https://doi.org/10.14456/apst.2020.25
Keywords:
Cryopreservation Genetic stability Seeds Rhynchostylis gigantea

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Duangrath Jariyajirawattana
1Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
Sumontip Bunnag
Applied Taxonomic Research Center (ATRC), Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand

Abstract

Orchids are highly important and valuable plants due to their diversity and the beauty of their flowers. Presently, there are many orchid species, which are going to become extinct due to environmental changes and over-collection for commercial uses. Cryopreservation is an alternative method for the storage of plant genetic resources. The aim of this study was to evaluate the effectiveness of the vitrification method for the cryopreservation of Rhynchostylis gigantea seeds. The seeds were pre-cultured for 24 h in a liquid New Dogashima (ND) medium, which had been supplemented with 0.5 M sucrose; incubated with a mixture of 0.4 M sucrose and 2 M glycerol for 30 min; and then finally dehydrated with plant verification solution 2 (PVS2) for 0-120 min at 25 ˚C prior to storage in liquid nitrogen (LN) for 24 h. The orchid seeds were successfully preserved by the vitrification method. The results found that the optimal dehydration time in PVS2 had been 30 min, which gave the highest recovery rate of 61% after culturing the seeds on ND medium for 30 d. The results of genetic stability assessment revealed that when comparing the cryopreserved and non-cryopreserved seeds, the Random amplified polymorphic DNA (RAPD) patterns had been similar. A total of 92.5% of the RAPD primers had given a high SI value, which ranged between 0.9 and 1.0 and indicated that the genetic stability of seeds stored in liquid nitrogen had been well-maintained.

Article Details

How to Cite
Jariyajirawattana, D., & Bunnag, S. (2020). The effects of vitrification method on the seed survival and genetic stability of Rhynchostylis gigantea (Lindl.) Ridl. Asia-Pacific Journal of Science and Technology, 25(03), APST–25. https://doi.org/10.14456/apst.2020.25
Issue
Vol. 25 No. 03 (2020): JUL-SEP
Section
Research Articles

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