QTL mapping specific to Thrips palmi resistance in Capsicum annuum
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Abstract
Capsicum, more commonly known as red pepper or chili pepper, is an important vegetable and spice throughout the world. Thrips are insects that cause highly devastating losses to Capsicum production, resulting in both direct and indirect damage. Some resistance to Thrips palmi (T. palmi) has been identified in the Capsicum species, with tests revealing that resistance to thrips is species specific. In this study, a Quantitative Trait Loci (QTL) analysis was performed to determine resistance against T. palmi in an F2 population derived from a cross between the highly resistant C. annuum AC 1979 and the highly susceptible C. annuum Berceo. In addition, a resistance test (Choice method) was used to study phenotypic data. One hundred sixty-one SNPs markers were used to construct a linkage map. An F2 genetic linkage map, constructed with a JoinMap 4.0 program, consisted of 13 linkage groups with a total length of 783.84 cM. The interval mapping of the area under the disease progress curve (AUDPC) showed significance in the LG1 (Chromosome 3) and LG2 (Chromosome 12). The highly significant QTL in LG1 was located on the M238 SNP marker with about 12.2% explained phenotypic variance. The highly significant QTL in LG2 was located on the M171 SNP marker with an explained phenotypic variance of 8.9%. These two QTLs may play a role in T. palmi resistance in C. annuum. Moreover, these M238 and M171 SNP markers will be used in pepper marker-assisted breeding for T. palmi resistance.
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