Screening of mycoviruses from fungal isolates causing dirty panicle disease in rice seeds

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Published: Jul 30, 2021
DOI: https://doi.org/10.14456/apst.2021.51
Keywords:
Biocontrol Dirty rice panicle disease Mycovirus Phytopathogenic fungi

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Aditya R. Suharto
Natural Resource Management Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Piyanuch Janmol
Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Taweerat Vichitsoonthonkul
Biotechnology Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Lakha Salaipeth
LigniTech-Lignin Technology Research Group, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

Abstract

Dirty rice panicle disease, caused by various types of phytopathogenic fungi, results in the damage and deterioration of rice seeds. This disease is mainly controlled by using fungicides. Despite their usefulness, fungicides could pose potential risks to environmental pollution and health. Recently, researchers found that fungi can be infected by various types of mycoviruses that induce hypovirulence. This study aimed to acquire mycoviruses from rice dirty panicle fungi. Pathogenic fungi were isolated from rice fields (Pathum Thani 1 cultivar) in Pathum Thani, Phatthalung, and Chachoengsao province, Thailand. Of the 129 phytopathogenic fungi isolates, most of them were Fusarium spp. (44.17%), followed by Curluvaria spp. (20.93%), Aspergillus spp. (11.62%), and Penicillium spp. (7.75%), and some isolates (15.53%) could not be identified based on their morphology and sporulation. Mycoviruses were screened, and 10 fungal isolates showed viral-double stranded RNA (dsRNA) profiles on agarose gel electrophoresis. The estimated size of the mycovirus varied from 2 to 10 kb, and they were divided into 8 types based on their dsRNA profiles. Moreover, the identification of fungal isolates harbouring mycoviruses based on ITS regions was carried out. The results showed that 10 fungal isolates belonged to the phylum Ascomycota and were distributed in three orders: Pleosporales, Trichosphaeriales, and Hypocreales. This finding suggests the appearance of mycoviruses in various types of fungal isolates. Moreover, the use of mycoviruses as a potential candidate for biocontrol of phytopathogenic fungi should be a subject of further investigation.

Article Details

How to Cite
Suharto, A. R. ., Janmol, P. ., Vichitsoonthonkul, T. ., & Salaipeth, L. . (2021). Screening of mycoviruses from fungal isolates causing dirty panicle disease in rice seeds. Asia-Pacific Journal of Science and Technology, 26(03), APST–26. https://doi.org/10.14456/apst.2021.51
Issue
Vol. 26 No. 03 (2021): JUL-SEP (TSB-2020)
Section
Research Articles

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