Screening of fungi isolated from damaged plant materials for the production of lignocellulolytic enzymes with decolorizing ability
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Published:
Aug 5, 2021
Keywords:
Curvularia sp. Fusarium sp. Decolorization Food waste Lignocelluloytic enzyme Phanerochaete sp. Solid-state fermentation
Main Article Content
Abstract
Lignocellulolytic fungi are the major sources of enzymes, mainly cellulase, xylanase, and oxidative enzymes. In this study, three fungal species, identified as strains TV-1, PLS-21#7, and PSA-3, were isolated from damaged areas of infected plants. Based on morphological observation, the species were classified into Phanerochaete sp. TV-1, Curvularia sp. PLS21#7, and Fusarium sp. PSA-3, respectively. The fungi were grown on agar media containing carboxymethyl cellulose and Beechwood xylan, due to which clear zones surrounded the colonies on both agar media. The results indicate that strains Phanerochaete sp. TV-1, Curvularia sp. PLS21#7, and Fusarium sp. PSA-3 have cellulolytic and xylanolytic activities. The fungal isolates were grown on ground cassava pulp and durian peel at the temperature of 25 ℃ for ten days to determine their growth ability and enzyme production profile. Unlike durian peel, cassava pulp induced cellulase and xylanase synthesis for the three fungal strains. Although the crude enzyme of Phanerochaete sp. TV-1, grown on cassava pulp, showed higher cellulase and xylanase activities than those of Curvularia sp. PLS21#7, and Fusarium sp. PSA-3, the decolorizing ability against Indigo carmine was not different among the three strains (p-value>0.05), showing around 40-60% decolorization within three days. Our study demonstrates that the new Phanerochaete sp. TV-1, Curvularia sp. PLS21#7, and Fusarium sp. PSA-3 strains can be used as hosts for enzyme production in low-cost media. These enzymes can be further applied in biomass saccharification or treatment of synthetic dye-containing wastewater.
Article Details
How to Cite
Bundidamorn, D. ., Salaiphet, L. ., Vichitsoonthonkul, T. ., Ratanakhanokchai, K. ., & Phitsuwan, P. . (2021). Screening of fungi isolated from damaged plant materials for the production of lignocellulolytic enzymes with decolorizing ability . Asia-Pacific Journal of Science and Technology, 26(03), APST–26. https://doi.org/10.14456/apst.2021.54
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Research Articles
References
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[31] Balan DSL, Monteiro RTR. Decolorization of textile indigo dye by ligninolytic fungi. J Biotechnol. 2001;89:141-145.
[32] Miranda Rde C, Gomes Ede B, Pereira N, Marin-Morales MA, Machado KM, Gusmao NB. Biotreatment of textile effluent in static bioreactor by Curvularia lunata URM 6179 and Phanerochaete chrysosporium URM 6181. Bioresour Technol. 2013;142:361-367.
[33] Podgornik H, Poljanšek I, Perdih A. Transformation of Indigo carmine by Phanerochaete chrysosporium ligninolytic enzymes. Enzyme Microb Technol. 2001;29:166-172.
[2] Guerriero G, Hausman JF, Strauss J, Ertan H, Siddiqui KS. Destructuring plant biomass: focus on fungal and extremophilic cell wall hydrolases. Plant Sci. 2015;234:180-193.
[3] Dashtban M, Schraft H, Qin W. Fungal bioconversion of lignocellulosic residues; opportunities and perspectives. Int J Biol Sci. 2009;5:578-595.
[4] Alawlaqi MM, Alharbi AA. Exo and endoglucanase production by Curvularia affinis using bean (Phaseolus vulgaris L.) waste biomass. Bioresour Bioproc. 2020;7(6)1-11.
[5] Uzma F, Konappa NM, Chowdappa S. Diversity and extracellular enzyme activities of fungal endophytes isolated from medicinal plants of Western Ghats, Karnataka. Egyptian J Basic Appl Sci. 2019;3:335-342.
[6] Huang Y, Busk PK, Lange L. Cellulose and hemicellulose-degrading enzymes in Fusarium commune transcriptome and functional characterization of three identified xylanases. Enzyme Microb Technol. 2015; 73-74:9-19.
[7] DoboziLt MS, Szakacs G, Bruschi CV. Xylanase activity of Phanerochaete chrysosporium. Appl Environ Microbiol. 1992;58:3466-3471.
[8] Vázquez MA, Cabrera ECV, Aceves MA, Mallol JLF. Cellulolytic and ligninolytic potential of new strains of fungi for the conversion of fibrous substrates. Biotecnol Res Innov. 2019;3:177-186.
[9] Huy ND, Ha DTT, Khoo KS, Lan PTN, Quang HT, Loc NH, et al. Synthetic dyes removal by Fusarium oxysporum HUIB02 and stimulation effect on laccase accumulation. Environ Technol Innov. 2020; 19:101027.
[10] Xiros C, Katapodis P, Christakopoulos P. Factors affecting cellulose and hemicellulose hydrolysis of alkali treated brewers spent grain by Fusarium oxysporum enzyme extract. Bioresour Technol. 2011;102: 1688-1696.
[11] Przystas W, Zablocka-Godlewska E, Grabinska-Sota E. Efficacy of fungal decolorization of a mixture of dyes belonging to different classes. Braz J Microbiol. 2015;46:415-424.
[12] Przystas W, Zablocka-Godlewska E, Grabinska-Sota E. Efficiency of decolorization of different dyes using fungal biomass immobilized on different solid supports. Braz J Microbiol. 2018;49:285-295.
[13] Gusakov AV. Alternatives to Trichoderma reesei in biofuel production. Trends Biotechnol. 2011;29: 419-425.
[14] Harris PV, Xu F, Kreel NE, Kang C, Fukuyama S. New enzyme insights drive advances in commercial ethanol production. Curr Opin Chem Biol. 2014;19:162-170.
[15] Okal EJ, Aslam MM, Karanja JK, Nyimbo WJ. Mini review: advances in understanding regulation of cellulase enzyme in white-rot basidiomycetes. Microb Pathog. 2020;147:1-8.
[16] Srivastava N, Srivastava M, Mishra PK, Gupta VK, Molina G, Rodriguez-Couto S, et al. Applications of fungal cellulases in biofuel production: advances and limitations. Renew Sust Energ Rev. 2018;82: 2379-2386.
[17] Yoon LW, Ang TN, Ngoh GC, Chua ASM. Fungal solid-state fermentation and various methods of enhancement in cellulase production. Biomass Bioenergy. 2014;67:319-338.
[18] Febrinasari T, Tae H, Riki N, Yotsombat A, Goro T, Cahyanto MN, et al. Isolation of corncob xylan-degrading fungi and its application in xylobiose production. CMUJ Nat Sci. 2021;20(2):1-14.
[19] Ahirwar S, Soni H, Prajapati BP, Kango N. Isolation and screening of thermophilic and thermotolerant fungi for production of hemicellulases from heated environments. Mycology. 2017;8:125-134.
[20] Saroj P, Narasimhulu K. Characterization of thermophilic fungi producing extracellular lignocellulolytic enzymes for lignocellulosic hydrolysis under solid-state fermentation. Bioresour Bioproc. 2018;5(31): 1-14.
[21] Miller GL. Use of dinitrosaiicyiic acid reagent for determination of reducing sugar. Anal Chem. 1959;31: 426-428.
[22] Delila L, Susanti E, Sanjaya EH. Isolation and screening of indigenous fungus producing lignin peroxidase from the cocoa plantation in sepawon kediri regency Indonesia. Kne Life Sci. 2017;3:131-138.
[23] M’barek HN, Taidi B, Smaoui T, Aziz MB, Mansouri A, Hajjaj H. Isolation, screening and identification of ligno-cellulolytic fungi from northern central Morocco. Biotechnol Agron Soc Environ. 2019;23: 207-217.
[24] Trabelsi R, Sellami H, Gharbi Y, Krid S, Cheffi M, Kammoun S, et al. Morphological and molecular characterization of Fusarium spp. associated with olive trees dieback in Tunisia. 3 Biotech. 2017;7(28): 1-9.
[25] Teather RM, Wood PJ. Use of congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rument. Appl Environ Microbiol. 1982;43:777-780.
[26] Virunanon C, Ouephanit C, Burapatana V, Chulalaksananukul W. Cassava pulp enzymatic hydrolysis process as a preliminary step in bio-alcohols production from waste starchy resources. J Clean Prod. 2013; 39:273-279.
[27] Siwina S, Leesing R. Bioconversion of durian (Durio zibethinus Murr.) peel hydrolysate into biodiesel by newly isolated oleaginous yeast Rhodotorula mucilaginosa KKUSY14. Renew Energ. 2021;163:237-245.
[28] de Almeida MN, Falkoski DL, Guimarães VM, de Rezende ST. Study of gamba grass as carbon source for cellulase production by Fusarium verticillioides and its application on sugarcane bagasse saccharification. Ind Crop Prod. 2019;133:33-43.
[29] Xiros C, Topakas E, Katapodis P, Christakopoulos P. Evaluation of Fusarium oxysporum as an enzyme factory for the hydrolysis of brewer's spent grain with improved biodegradability for ethanol production. Ind Crop Prod. 2008;28:213-224.
[30] Brandt SC, Brognaro H, Ali A, Ellinger B, Maibach K, Ruhl M, et al. Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources. Biotechnol Biofuels. 2021;14(74):1-22.
[31] Balan DSL, Monteiro RTR. Decolorization of textile indigo dye by ligninolytic fungi. J Biotechnol. 2001;89:141-145.
[32] Miranda Rde C, Gomes Ede B, Pereira N, Marin-Morales MA, Machado KM, Gusmao NB. Biotreatment of textile effluent in static bioreactor by Curvularia lunata URM 6179 and Phanerochaete chrysosporium URM 6181. Bioresour Technol. 2013;142:361-367.
[33] Podgornik H, Poljanšek I, Perdih A. Transformation of Indigo carmine by Phanerochaete chrysosporium ligninolytic enzymes. Enzyme Microb Technol. 2001;29:166-172.