Bacterial biocontrol against Fusarium wilt in Pisang Awak (Namwa) Banana
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Published:
Sep 7, 2021
Keywords:
Microbiome Rhizosphere Bacillus Pseudomonas Foc
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Abstract
Pisang Awak is one of the most important banana cultivars due to its drought tolerance and nutritional value. Nonetheless, Pisang Awak is highly susceptible to Fusarium wilt caused by Fusarium oxysporum f.sp. cubense (Foc). Over the past decades, the use of biocontrol bacteria against Foc has shown promising results in both greenhouse and field trials. However, none of these previous studies demonstrated effective control of Fusarium wilt in Pisang Awak. We show that Pseudomonas and Bacillus strains isolated in Thailand can suppress Foc mycelial growth in vitro and significantly mitigate the severity of Fusarium wilt in Pisang Awak (Namwa) banana in a greenhouse experiment. With these bacterial treatments, Fusarium wilt incidences can be reduced by up to 70% and 89% in young and old banana plants, respectively. We have also reported the first bacterial community analysis of Pisang Awak rhizospheres in response to Foc and bacterial biocontrol. The abundance of several bacterial families such as Rhodospirillaceae, Bradyrhizobiaceae and Hyphomicrobiaceae increased in rhizospheres inoculated with Foc and decreased in rhizospheres treated with biocontrol. These families were previously shown to be associated with Foc suppressive soil. These results provide the basis for the development of bacterial biocontrol products against Foc in Pisang Awak banana.
Article Details
How to Cite
Mahachai, P., Meesungnoen, O. ., Wattanachaiyingcharoen, W. ., & Subsoontorn, P. . (2021). Bacterial biocontrol against Fusarium wilt in Pisang Awak (Namwa) Banana. Asia-Pacific Journal of Science and Technology, 26(03), APST–26. https://doi.org/10.14456/apst.2021.57
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Research Articles
References
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[27] Rames EK, Pattison AB, Czislowski E, Smith MK. Soil microbial community changes associated with ground cover management in cultivation of Ducasse banana (Musa sp. ABB, Pisang Awak subgroup) and suppression of Fusarium oxysporum. Australas Plant Pathol. 2018;47(4):449-462.
[28] Zhang J, Bei S, Li B, Zhang J, Christie P, Li X. Organic fertilizer, but not heavy liming, enhances banana biomass, increases soil organic carbon, and modifies soil microbiota. Appl Soil Ecol. 2019;136:67-79.
[29] Kaushal M, Swennen R. Unlocking the microbiome communities of banana (Musa spp.) under disease stressed (Fusarium wilt) and non-stressed conditions. Microorganisms. 2020;8(443):1-19.
[30] Klein E, Ofek M, Katan J, Minz D, Gamliel A. Soil suppressiveness to Fusarium disease: shifts in root microbiome associated with reduction of pathogen root colonization. Phytopathology. 2013;103(1):23-33.
[31] Shaffer JP, U’Ren JM, Gallery RE, Baltrus DA, Arnold AE. An endohyphal bacterium (Chitinophaga, Bacteroidetes) alters carbon source use by Fusarium keratoplasticum (F. solani species complex, Nectriaceae). Front Microbiol. 2017;8:350.
[2] Dita M, Barquero M, Heck D, Mizubuti ESG, Staver CP. Fusarium wilt of banana: current knowledge on epidemiology and research needs toward sustainable disease management. Front Plant Sci. 2018;871:1-21.
[3] Warman NM, Aitken EAB. The movement of Fusarium oxysporum f.sp. Cubense (sub-tropical race 4) in susceptible cultivars of banana. Front Plant Sci. 2018;871:1-9.
[4] Bubici G, Kaushal M, Prigigallo MI, Cabanás CGL, Mercado-Blanco J. Biological control agents against Fusarium wilt of banana. Front Microbiol. 2019;10:616.
[5] Raza W, Ling N, Zhang R, Huang Q, Xu Y, Shen Q. Success evaluation of the biological control of Fusarium wilts of cucumber, banana, and tomato since 2000 and future research strategies. Crit Rev Biotechnol. 2017;37(2):202-212.
[6] Ravi I, Uma S, Vaganan MM, Mustaffa MM. Phenotyping bananas for drought resistance. Front Physiol. 2013;4:1-15.
[7] DOA Thailand [Internet]. 2019 [cited 26 Feb 2021]. Available from: http://www.agriinfo.doae.go.th/ year62/ plant/ rortor/ fruit.pdf
[8] Guo L, Yang L, Liang C, Wang G, Dai Q, Huang J. Differential colonization patterns of bananas (Musa spp.) by physiological race 1 and race 4 isolates of Fusarium oxysporum f.sp. cubense. J Phytopathol. 2015;163(10):807-817.
[9] Xue C, Penton CR, Shen Z, Zhang R, Huang Q, Li R, et al. Manipulating the banana rhizosphere microbiome for biological control of Panama disease. Sci Rep. 2015;5:1-11.
[10] Rames EK, Pattison AB, Czislowski E, Smith MK. Soil microbial community changes associated with ground cover management in cultivation of Ducasse banana (Musa sp. ABB, Pisang Awak subgroup) and suppression of Fusarium oxysporum. Australas Plant Pathol. 2018;47(4):449-462.
[11] Kaushal M, Swennen R. Unlocking the microbiome communities of banana (Musa spp.) under disease stressed (Fusarium wilt) and non-stressed conditions. Microorganisms. 2020;8(443):1-19.
[12] Vlassak K, Van Holm L, Duchateau L, Vanderleyden J, De Mot R. Isolation and characterization of fluorescent Pseudomonas associated with the roots of rice and banana grown in Sri Lanka. Plant Soil. 1992;145:51-63.
[13] Morton DJ, Stroube WH. Antagonistic and stimulatory effects of soil microorganisms upon Sclerotium rolfsii. Phytopath. 1955;45(8):417-420.
[14] Vincent JM. Distortion of fungal hyphæ in the presence of certain inhibitors. Nature. 1947;159:850.
[15] Sivamani E, Gnanamanickam SS. Biological control of Fusarium oxysporum f.sp. cubense in banana by inoculation with Pseudomonas fluorescens. Plant Soil. 1988;107:3-9.
[16] Moore N, Pegg K, Allen R, Irwin J. Vegetative compatibility and distribution of Fusarium oxysporum f.sp. cubense in Australia. Aust J Exp Agric. 1993;33(6):797-802.
[17] R Core-Team. R: a language and environment for statistical computing [Internet]. 2017 [cited 26 Feb 2021]. Available from: https://www.R-project.org/. Available from: https://www.r-project.org/
[18] Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol. 2019;37(8): 852-857.
[19] DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, et al. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol. 2006;72(7): 5069-5072.
[20] Prathuangwong S, Athinuwat D, Chuaboon W, Chatnaparat T, Buensanteai N. Bioformulation Pseudomonas fluorescens SP007s against dirty panicle disease of rice. African J Microbiol Res. 2013; 7(47):5274-5283.
[21] Chaisue S, Poralokanon P, Chuaboon W, Athinuwat D. Pseudomonas fluorescens produced polysaccharides enhance growth of cassava plant and control root and tuber rot. Sci Technol J. 2016;561-572.
[22] Bunkoed W, Chaijuckam P, Kasam S, Yhamsoongnern J, Kladsuwan L, Choorin M, et al. Effect of antibiotic from bacterial antagonist, SP007s, on growth inhibition of E. turcicum. In: Proceedings of 53rd Kasetsart University Annual Conference;2015 Feb 3-6; Thai National AGRIS Centre, Office of The University Library Kasetsart University. Bankok; 2015. p. 263-270.
[23] Gholami A, Shahsavani S, Nezarat S. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. World Acad Sci Eng Technol. 2009;37:19-24.
[24] Nezarat S, Gholami A. Screening plant growth promoting rhizobacteria for improving seed germination, seedling growth and yield of maize. Pakistan J Biol Sci. 2009;12(1):26-32.
[25] Saravanan T, Muthusamy M, Marimuthu T. Pseudomonas fluorescens induced enzymological changes in banana roots (Cv. Rasthali) against Fusarium wilt disease. Plant Pathol J. 2004;3(2):72-80.
[26] Akila R, Rajendran L, Harish S, Saveetha K, Raguchander T, Samiyappan R. Combined application of botanical formulations and biocontrol agents for the management of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt in banana. Biol Control. 2011;57(3):175-183.
[27] Rames EK, Pattison AB, Czislowski E, Smith MK. Soil microbial community changes associated with ground cover management in cultivation of Ducasse banana (Musa sp. ABB, Pisang Awak subgroup) and suppression of Fusarium oxysporum. Australas Plant Pathol. 2018;47(4):449-462.
[28] Zhang J, Bei S, Li B, Zhang J, Christie P, Li X. Organic fertilizer, but not heavy liming, enhances banana biomass, increases soil organic carbon, and modifies soil microbiota. Appl Soil Ecol. 2019;136:67-79.
[29] Kaushal M, Swennen R. Unlocking the microbiome communities of banana (Musa spp.) under disease stressed (Fusarium wilt) and non-stressed conditions. Microorganisms. 2020;8(443):1-19.
[30] Klein E, Ofek M, Katan J, Minz D, Gamliel A. Soil suppressiveness to Fusarium disease: shifts in root microbiome associated with reduction of pathogen root colonization. Phytopathology. 2013;103(1):23-33.
[31] Shaffer JP, U’Ren JM, Gallery RE, Baltrus DA, Arnold AE. An endohyphal bacterium (Chitinophaga, Bacteroidetes) alters carbon source use by Fusarium keratoplasticum (F. solani species complex, Nectriaceae). Front Microbiol. 2017;8:350.