Rapid detection of Ralstonia syzygii subsp. syzygii on cloves vascular disease and identification potential alternate host
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Abstract
Vascular diseases caused by bacterial is one of the main problems of clove production in Central Sulawesi, Indonesia. In this study, rapid detection using polymerase chain reaction (PCR) was used to detect and characterize the causal agent of the diseases. The pathogenicity of pathogens was tested on clove, guava, banana, chili, tomato, heliconia, and three weeds that grow on clove plantations. Furthermore, the plants were checked using PCR detection to determine potential alternate hosts if they can be a pathogen-host without any symptoms. A specific primer for Ralstonia syzigii subsp. syzygii was used to amplify the bacterial pathogens in plant Deoxyribonucleic acid (DNA). PCR detection showed that all plant parts collected from clove plantations were infected by R. syzigii on DNA fragments (378 bp in size). Pathogenicity test indicates vascular disease symptoms on cloves, and there were no symptoms on other plants tested. Interestingly, PCR detection showed that the DNA of all plants tested was associated with R. syzigii. This study figured out that vascular diseases on clove plantations in Central Sulawesi Indonesia are associated with R. syzigii. In addition, this study also found a potential alternate asymptomatic host for R. syzigii on several plants.
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