Biomass and biohydrogen production by unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261 using frozen food industrial wastewater

Article Sidebar

PDF
Published: Feb 2, 2022
DOI: https://doi.org/10.14456/apst.2022.19
Keywords:
Biohydrogen Biomass Chlorella vulgaris var. vulgaris TISTR 8261 Food industrial wastewater

Main Article Content

Samart Taikhao
Faculty of Science and Technology, Rajamangala University of Technology Suvarnabhumi, Nonthaburi, Thailand
Saranya Phunpruch
Department of Biology, School of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand

Abstract

Biohydrogen production by green algal biomass is promising method for sustainable H2 production and bioenergy recovery. In this approach, green algae convert organic and inorganic substances (used as the sole source of carbon and electrons) in wastewater into H2. In this study, biomass and H2 production from the unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261, cultivated in frozen food industrial wastewater, was investigated. The results revealed that growth rate of algal cells cultivated in treated wastewater was significantly higher than that in untreated wastewater but lower than that in the synthetic control Tris acetate phosphate (TAP) medium. In addition, the cells grown in treated wastewater exhibited a high ability to remove nitrate, nitrite, phosphate, and sulfate from the water. Furthermore, algal cells were cultured with various concentrations of sodium acetate (0-17.4 mM); the optical density of the cultures at 750 nm increased with increase in acetate concentration. Cell growth in treated wastewater supplemented with 17.4 mM sodium acetate was similar to that in TAP medium. The highest H2 production of 12.87 ± 0.58 µmolH2 mg Chl a-1 was observed in cells incubated in treated wastewater supplemented with 17.4 mM sodium acetate; this yield was higher than that obtained from cells incubated in nitrogen-free TAP medium. The results of this study support the potential use of wastewater for biomass and biohydrogen production by C. vulgaris var. vulgaris TISTR 8261.

Article Details

How to Cite
Taikhao, S., & Phunpruch, S. (2022). Biomass and biohydrogen production by unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261 using frozen food industrial wastewater. Asia-Pacific Journal of Science and Technology, 27(01), APST–27. https://doi.org/10.14456/apst.2022.19
Issue
Vol. 27 No. 01 (2022): JAN-FEB
Section
Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Sui J, Chen Z, Wang C, Wang Y, Liu J, Li W. Efficient hydrogen production from solar energy and fossil fuel via water-electrolysis and methane-steam-reforming hybridization. Appl Energy. 2020;276:1-11.

Hu J, Cao W, Guo L. Directly convert lignocellulosic biomass to H2 without pretreatment and added cellulase by two-stage fermentation in semi-continuous modes. Renew Energy. 2021;170:866-874.

Eroglu E, Melis A. Microalgal hydrogen production research. Int J Hydrogen Energy. 2016;41:12772-12798.

Nirmalakhandan N, Selvaratnam T, Gedera HS, Tchinda D, Arachchige AI, Pedige DH, et al. Algal wastewater treatment: Photoautotrophic vs. mixotrophic processes. Algal Res. 2019;41:1-9.

Chiu SY, Kao CY, Chen TY, Chang, YB, Kuo CM, Lin CS. Cultivation of microalgal Chlorella for biomass and lipid production using wastewater as nutrient resource. Bioresour Technol 2015;184:179-189.

Liu S, Gifuni I, Mear H, Frappart M, Couallier E. Recovery of soluble proteins from Chlorella vulgaris by bead-milling and microfiltration: Impact of the concentration and the physicochemical conditions during the cell disruption on the whole process. Process Biochem. 2021;108:34-47.

Daneshvar E, Ok YS, Tavakoli S, Sarkar B, Shaheen SM, Hong H, et al. Insights into upstream processing of microalgae: a review. Bioresour Technol. 2021;329(1):1-16.

Cornelius HO, Vu T, Schmiedhofer C, Vielnascher R, Dielacher M, Sachs V, et al. Cultivation of heterotrophic algae on enzymatically hydrolyzed municipal food waste. Algal Res. 2020;50:1-10.

Farooq W, Lee YC, Ryu BG, Kim BH, Kim HS, Choi YE, et al. Two stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity. Bioresour Technol. 2013;132:230-238.

Zhu L, Wang Z, Takala J, Hiltunen E, Qin L, Xu Z, et al. Scale up potential of cultivating Chlorella zofingiensis in piggery wastewater for biodiesel production. Bioresour Technol. 2013;137:318-325.

Amutha KB, Murugesan A. Biological hydrogen production by the algal biomass Chlorella vulgaris MSU 01 strain isolated from pond sediment. Bioresour Technol. 2011;102:194-199.

Faraloni C, Ena A, Pintucci C, Torzillo G. Enhanced hydrogen production by means of sulfur-deprived Chlamydomonas reinhardtii cultures grown in pretreated olive mill wastewater. Int J Hydrogen Energy. 2011;36:5920-5931.

Chen M, Zhang L, Li S, Chang S, Wang W, Zhang Z, et al. Characterization of cell growth and photobiological H2 production of Chlamydomonas reinhardtii in ASSF industry wastewater. Int J Hydrogen Energy. 2014;39:13462-13467.

Rattana S. Hydrogen production by unicellular green alga Scenedesmus sp. KMITL-O1. [Master’s thesis]. Bangkok: King Mongkut's Institute of Technology Ladkrabang; 2011.

American Public Health Association. Standard methods for the examination of water and wastewater. 20th ed. Washington: American Public Health Association, American Water Works Association and Water Environmental Federation;1998.

Harris EH. The Chlamydomonas sourcebook: a comprehensive guide to biology and laboratory use. 1st ed. San Diego: Academic Press; 1989.

Lee YK, Shen H. Basic culturing techniques. In: Richmond A, editor. Handbook of microalgal culture. 1st ed. Oxford: Blackwell Publishing Ltd; 2004. p. 40-56.

Taikhao S, Junyapoon S, Incharoensakdi A, Phunpruch S. Factors affecting biohydrogen production by unicellular halotolerant cyanobacterium Aphanothece halophytica. J Appl Phycol. 2013;25:575-585.

Taziki M, Ahmadzadeh H, Murry M, Lyon SR. Nitrate and nitrite removal from wastewater using algae. Curr Biotechnol. 2015;4:1-15.

Pittman JK, Dean AP, Osundeko O. The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol. 2011;102:17-25.

Sun X, Wang C, Li Z, Wang W, Tong, Y, Wei J. Microalgal cultivation in wastewater from the fermentation effluent in Riboflavin (B2) manufacturing for biodiesel production. Bioresour Technol. 2013;143:499-504.

Aslan S, Kapdan IK. Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae. Ecol Eng. 2006;28:64-70.

Liu JZ, Yin JY, Han HF, Ge YM, Wang ZY, Bao XY, et al. Enhancements of lipid productivity and phosphorus utilization efficiency of Chlorella pyrenoidosa by iron and acetate supplements in actual municipal wastewater. Renew Energy. 2021;170:927-935.

Garcia PO, Escalante FME, de-Bashan LE, Bashan Y. Heterotrophic cultures of microalgae: metabolism and potential products. Water Res. 2011;45:11-36.

Arroyo HT, Wei W, Ruan R, Hu B. Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass Bioenergy. 2011;35:2245-2253.

Hongyang S, Yalei Z, Chunmin W, Xuefei Z, Jinpeng L. Cultivation of Chlorella pyrenoidosa in soy bean processing wastewater. Bioresour Technol. 2011;102:9884-9890.

Srirangan K, Pyne ME, Chou CP. Biochemical and genetic engineering strategies to enhance hydrogen production in photosynthetic algae and cyanobacteria. Bioresource Technol. 2011;102:8589-8604.

Fouchard S, Hemschemeier A, Caruana A, Pruvost K, Legrand J, Happe T, et al. Autotrophic and mixotrophic hydrogen photoproduction in sulfur-deprived Chlamydomonas cells. Appl Environ Microbiol. 2005;71:6199-6205.

Wang H, Xiong H, Hui Z, Zeng X. Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresour Technol. 2012;104:215-220.