Amplified fragment length polymorphism for identification of Habenaria and Pecteilis

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Nipon Kitidee
Nuttha Potapohn
Weenun Bundithya


Habenaria and Pecteilis are terrestrial orchids that belong to the same subtribe. These two genera exhibit some similar features and are sometimes mistaken for the same genera. Nowadays, the molecular marker method can be used to elucidate the relationship between plants which are different in terms of morphology, but molecular markers can also show similarities between and within genera. There are several molecular markers which can be employed. One of the popular methods is the amplified fragment length polymorphism (AFLP) technique. This technique was used to classify 30 samples of Habenaria and Pecteilis. Among 64 combinations of 8 EcoRI primers and 8 Tru9Ⅰ primers, four primer combinations (E-ATT/M-CAC), (E-AGT/M-CTG), (E-ACA/M-CAC) and (E-ACC/M-CAC) produced clear and polymorphic bands. Similarity coefficients between species indicated that H. erichmichaelii and H. carnea had the closest genetic relationship of 0.60. All samples could be divided into 2 groups. Group 1 consisted of 27 samples of Habenaria with small to large colorful flowers. These samples could be further separated into 3 subgroups, with subgroup 1.1 consisting of Habenaria, which have big and colorful flowers, subgroup 1.2 consisted of Habenaria, which has white, light green or green flowers and subgroup 1.3 consisted of Habenaria, which had small yellow and pink flowers. Group 2 consisted of 3 samples of Pecteilis with large white and yellow flowers. The results indicated that this technique could be used to distinguish Habenaria and alliances.

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How to Cite
Kitidee, N., Potapohn, N., & Bundithya, W. (2023). Amplified fragment length polymorphism for identification of Habenaria and Pecteilis. Asia-Pacific Journal of Science and Technology, 28(05), APST–28.
Research Articles


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