In-vitro evaluation of actinomycetes with plant growth-promoting properties

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Published: Mar 8, 2022
DOI: https://doi.org/10.14456/apst.2022.33
Keywords:
Actinomycetes Biofertilizer Plant growth-promoting bacteria

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Napawit Nonthakaew
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
Watanalai Panbangred
King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Wisuwat Songnuan
Department of Plant Science, Faculty of Science, Mahidol University, Bangkok, Thailand
Bungonsiri Intra
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand

Abstract

The aim of this study was to evaluate actinomycetes for plant growth-promoting (PGP) traits using in vitro experiments. Actinomycetes were isolated from two different soil rhizospheres and subjected to dual culture experiments in order to determine antimicrobial activity. Subsequently, selected isolates with potent antimicrobial activity were examined further for their ability to biosynthesize indole-3-acetic acid (IAA) and siderophores, solubilize inorganic phosphate, and produce ammonia. Results showed that these strains produced 38.73-211.35 ng/mL IAA in Tryptic Soy Broth (TSB), and solubilized 116.20-319.33 µg/mL inorganic phosphate in National Botanical Research Institute's phosphate growth medium (NBRIP) broth. Additionally, all selected actinomycetes produced siderophores in chrome azurol S (CAS) agar and ammonia in peptone water. Five isolates showed promise as plant growth promoters, with isolates SNN045, SNN056, and SNN059 being the most plausible candidates for agricultural applications.

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How to Cite
Nonthakaew, N., Panbangred, W., Songnuan, W., & Intra, B. (2022). In-vitro evaluation of actinomycetes with plant growth-promoting properties. Asia-Pacific Journal of Science and Technology, 27(02), APST–27. https://doi.org/10.14456/apst.2022.33
Issue
Vol. 27 No. 02 (2022): MAR-APR (TSB Online 2021)
Section
Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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