Development of gold nanoparticles-based lateral flow sandwich assay for colorimetric and electrochemical detection of bacterial infection biomarker
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Abstract
In this study, two distinct technologies were used for quantitative and qualitative analysis of bacterial infection (BI) specific procalcitonin (PCT) biomarker. lateral flow assay (LFA) strips polyclonal antibody-gold nanoparticles ((pAb-AuNP)/ monoclonal antibody (mAb)/PCT) with the same PCT concentration are used as novel analytical performance on both platforms, and their results are investigated based on the existence of the test line bands. The authors present modified LFA strips (pAb-AuNP/mAb/PCT) for electrochemical studies using electrochemical impedance spectroscopy (EIS). A red band appeared on the test line, implying excellent outcomes. The limit of detection (LOD) for qualitative PCT detection using this LFA has been observed to be 1 ng/mL. The results of LFA- based test lines are validated further using quantitative colorimetric techniques. It demonstrates that the concentration used in qualitative LFA yields positive results and demonstrates a good linear correlation between visually detected ranges of 2 to 60 ng/mL. On the other hand, the quantitative electrochemical method, has a much higher sensitivity for the detection of PCT, with a linear range of 1 to 60 ng/mL, and 1 ng/mL LOD is observed. Furthermore, an LFA-based strip electrode was used in quantitative electrochemical measurements without any electrochemically active surface modifications, ensuring a simple and fast procedure. The quantitative platforms for PCT detection presented not only provide simple, rapid, and impacted methods, but they often can enhance sensing technology in other areas of health diagnostic research.
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