Genetic variation of wild Bulbophyllum reclusum Seidenf. in Northeast Thailand based on chloroplast matK sequence analysis
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Abstract
Bulbophyllum reclusum is one of the endangered wild orchid species in Thailand, and its genetic variation has never been elucidated using molecular techniques. The present study is the first to provide genetic variation information of wild B. reclusum collected from Northeast Thailand using the chloroplast maturase K (matK) gene as a marker. Based on the matK sequence analysis of 43 orchid samples from Sakon Nakhon and Ubon Ratchathani Provinces, 25 variable nucleotide sites were detected. There was no genetic variation in the samples collected from Sakon Nakhon Province. In contrast, high levels of intra- and inter-population genetic variation were observed in the samples collected from Ubon Ratchathani Province. However, the genetic differences between the populations were not significant and in a phylogenetic analysis, they clustered as a monophyletic group.
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