1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing endophytic bacteria prolong vase life of cut roses
Article Sidebar
Main Article Content
Abstract
Cultivating rose as a cut flower is a profitable vending business. Preserving cut roses by keeping them fresh and long-lasting is difficult. One of the factors that shortens their lifespan is ethylene exposure. However, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase produced by some plant growth-promoting bacteria converts ACC, an intermediate precursor of ethylene biosynthesis, to ammonia and alpha-ketobutyrate. This study aims to evaluate the ACC deaminase activity of endophytic bacteria in extending the lifespan of cut roses. The isolates were screened on the Dworkin and Foster (DF) medium containing 0.25 mM ACC (ACC-DF) and tested for their efficiency in decreasing ACC by a colorimetric ninhydrin assay. All selected isolates reduced ACC concentration in the culture medium. Acinetobacter sp. SWUy05 could colonize in the stem of cut roses and significantly retarded bud opening and petal wilting or discoloration. Therefore, Acinetobacter sp. SWUy05 is a promising ACC deaminase endophytic bacterium, suitable for extending the vase life of cut rose.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
In BC, Ha STT, Lee YS, Lim JH. Relationships between the longevity, water relations, ethylene sensitivity, and gene expression of cut roses. Postharvest Biol Tec. 2017;131:74-83.
Mladenovic E, Cukanovic J, Tanjga B, Pavlovic L, Hiel K, Miric M. Selection of garden rose cultivars for use as a cut flower. Genetika. 2018;50:495-502.
Khan AA. ACC-derived ethylene production, a sensitive test for seed vigor. J Am Soc Hort Sci. 1994;119:1083-1090.
Gamalero E, Glick BR. Mechanisms used by plant growth-promoting bacteria. In: Maheshwari D, editor. Bacteria in Agrobiology: Plant Nutrient Management, Berlin: Springer; 2011. p. 17-46.
Gepstein S, Glick BR. Strategies to ameliorate abiotic stress-induced plant senescence. Plant Mol Biol. 2013; 82:623-633.
Overbeek JHM, Woltering EJ. Synergistic effect of 1-aminocyclopropane-1-carboxylic acid and ethylene during senescence of isolated carnation petals. Physiol Plant. 1990;79:368-376.
Olanrewaju OS, Glick BR, Babalola OO. Mechanisms of action of plant growth promoting bacteria. World J Microbiol Biotechnol. 2017;33:197.
Arshad M, Saleem M, Hussain S. Perspectives of bacterial ACC-deaminase in phytoremediation. Trends Biotechnol. 2007;25:356-362.
Glick BR. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol Res. 2014;169:30-39.
Ali S, Charles TC, Glick BR. Delay of flower senescence by bacterial endophytes expressing 1-aminocyclopropane-1-carboxylate deaminase. J Appl Microbiol. 2012;113:1139-1144.
Wagstaff C, Chanasut U, Harren FJM, Laarhoven LJ, Thomas B, Rogers HJ, et al. Ethylene and flower longevity in Alstroemeria: relationship between tepal senescence, abscission and ethylene biosynthesis. J Exp Bot. 2005;56:1007-1016.
Sudaria MA, Uthairatanakij A, Nguyen HT. Postharvest quality effects of different vaselife solutions on cut rose (Rosa hybrida L.). Int J Agric Life Sci. 2017;1:12-20.
Rafi ZN, Ramezanian A. Vase life of cut rose cultivars ‘Avalanche’ and ‘Fiesta’ as affected by nano-silver and S-carvone treatments. S Afr J Bot. 2013;86:68-72.
Elgimabi MENE. Vase life extension of rose cut flowers (Rosa Hybirida) as influenced by silver nitrate and sucrose pulsing. Am J Agric Biol Sci. 2011;6:128-133.
Dworkin M, Foster JW. Experiments with some microorganisms which utilize ethane and hydrogen. J Bacteriol. 1958;75:592-601.
Ali SZ, Sandhya V, Rao LV. Isolation and characterization of drought-tolerant ACC deaminase and exopolysaccharide-producing fluorescent Pseudomonas sp. Ann Microbiol. 2014;64:493-502.
Penrose DM, Glick BR. Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria. Physiol Plant. 2003;118:10-15.
Li Z, Chang S, Lin L, Li Y, An Q. A colorimetric assay of 1-aminocyclopropane-1-carboxylate (ACC) based on ninhydrin reaction for rapid screening of bacteria containing ACC deaminase. Lett Appl Microbiol. 2011;53:178-185.
Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, et al. Introducing EzBiocloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol. 2017;67:1613-1617.
Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10:512-526.
Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870-1874.
Janda JM, Abbott SL. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: Pluses, perils, and pitfalls. J Clin Microbiol. 2007;45:2761-2764.
Chamani E, Wagstaff C. Effects of postharvest relative humidity and various re-cutting on vase life of cut rose flowers. Int J Postharvest Technol Innov. 2019;6:70-82.
Horibe T, Makita M. Promotion of flower opening in cut rose cultivars by 1-naphthaleneacetic acid treatment. Ornam Hortic. 2021;27:314-319.
Balas J, Coronado PAG, da-Silva JAT, Jayatilleke MP. Supporting post-harvest performance of cut flowers using fresh-flower-refreshments and other vase-water-additives. In: Silva JAT, editor. Floriculture, ornamental and plant biotechnology: advances and topical issues. Isleworth: Global Science Books; 2006. p. 612-629.
Ansari NA, Zangeneh M. Effects of cultivar, harvesting date and chemical treatments on the quality and soluble carbohydrate contents in rose (Rosa hybrida). Flori Ornam Biotech. 2008;2:1-4.
Nguyen TK, Lim JH. Do eco-friendly floral preservative solutions prolong vase life better than chemical solutions? Horticulturae. 2021;7:415.
Kosugia Y, Shibuya K, Tsurunoa N, Iwazakia Y, Mochizukib A, Yoshioka T, et al. Expression of genes responsible for ethylene production and wilting are differently regulated in carnation (Dianthus caryophyllus L.) petals. Plant Sci. 2000;158:139-145.
Sornchai P, van-Doorn WG, Imsabai W, Burns P, Chanprame S. Dendrobium orchids carrying antisense ACC oxidase: small changes in flower morphology and a delay of bud abortion, flower senescence, and abscission of flowers. Transgenic Res. 2020;29:429-442.
Aida R, Yoshida T, Ichimura K, Goto R, Shibata M. Extension of flower longevity in transgenic torenia plants incorporating ACC oxidase transgene. Plant Sci. 1998;138:91-101.
World Health Organization. Guidelines for drinking-water quality Vol I. Geneva: WHO; 2008.
Kuan KB, Othman R, Rahim KA, Shamsuddin ZH. Plant growth-promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilization of maize under greenhouse conditions. Plos One. 2016;11:e0152478.
Huang H, Huang M, Fan G, Liu X, Wang J, Duan Q, et al. Isolation and characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing plant growth-promoting rhizobacteria from carnation soil and roots. Afr J Microbiol Res. 2013;7:5664-5668.
Zhang YF, He LY, Chen ZJ, Zhang WH, Wang QY, Qian M, et al. Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape. J Hazard Mater. 2011;186:1720-1725.