Molecular markers for resistant starch content in Thai rice

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Published: Sep 28, 2023
DOI: https://doi.org/10.14456/apst.2023.101
Keywords:
Molecular marker Resistant starch RM marker SBEIIb SSIIIa Waxy

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Saran Cheenacharoen
Department of Biology, Faculty of Science and Technology, Chiang Mai Rajabhat University, Chiang Mai, Thailand
Sirima Suwarat
Program in Genetics, Faculty of Science, Maejo University, Chiang Mai, Thailand
Varaporn Saengtong
Program in Genetics, Faculty of Science, Maejo University, Chiang Mai, Thailand
Krisana Lanumteang
Program in Statistics, Faculty of Science, Maejo University, Chiang Mai, Thailand
Wiwat Wangcharoen
Program in Food Science and Technology, Faculty of Engineering and Agro-Industry, Maejo University, Chiang Mai, Thailand
Kewalin Chainurak
Program in Agronomy, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Varinee Prachakbunchetsad
Program in Agronomy, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Tararuk Arthan
Program in Agronomy, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Yuppayao Kophimai
Program in Genetics, Faculty of Science, Maejo University, Chiang Mai, Thailand

Abstract

Resistant starch (RS) is the starch that is not digested in the gastrointestinal tract. It is fermented to become a prebiotic that is beneficial to human health. This research aimed to find molecular markers linked to RS content in rice. The correlations between RS content and molecular markers specific to SSIIIa, SBEIIb and Waxy genes, which have been reported to affect RS content, were tested in an F2 population from two crosses_-RD-Maejo 2  RD43, which are glutinous and non-glutinous rice respectively, and Pathum Thani 1  Jao Leuang, which are both non-glutinous rice but have different amylose contents. The results showed that the RS content of 100 F2 plants from RD-Maejo 2  RD43 significantly correlated with the marker specific to starch type in the Waxy gene (r = 0.704, p < 0.001), and non-glutinous rice contained more RS content than glutinous rice. Likewise, the RS content of 92 F2 plants from Pathum Thani 1  Jao Leuang significantly correlated with the marker specific to SNP in exon 10 of the Waxy gene (r = 0.682, p < 0.001).  Rice with the high amylose allele from Jao Leuang had more RS content than rice with the low amylose allele from Pathum Thani 1. Positive correlation between RS content and amylose content was revealed (r = 0.837, p < 0.001). The results indicate that amylose content correlates with RS content and molecular markers of the Waxy gene can be used for rice screening when high RS content is concerned.

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How to Cite
Cheenacharoen, S. ., Suwarat, S. ., Saengtong, V. ., Lanumteang, K. ., Wangcharoen, W. . ., Chainurak, K. ., Prachakbunchetsad, V. ., Arthan, T. ., & Kophimai, Y. (2023). Molecular markers for resistant starch content in Thai rice. Asia-Pacific Journal of Science and Technology, 28(06), APST–28. https://doi.org/10.14456/apst.2023.101
Issue
Vol. 28 No. 06 (2023): NOV-DEC
Section
Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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