Ameliorative activity of α-tocopherol against potassium bromate-induced reproductive functional and morphological impairments in male rats
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Abstract
α-tocopherol (AT) acts as oxidative radical scavenger and protects against toxicants that impair reproductive functions and structures. Potassium bromate (PB) exhibits toxic effects on tissues via generation of reactive oxygen species (ROS) and impairment of antioxidant system. This study was aimed to evaluate the ameliorative effect of AT on PB-induced reproductive impairments in rats. Twenty male rats (150-170 g) were equally divided into four groups: group 1 (Control - Distilled water); group 2 (50 mg/kg AT only); group 3 (50 mg/kg AT + 25 mg/kg PB co-treated) and group 4 (25 mg/kg PB only). All treatments were done daily and orally for 28 days except PB (administered as single dose intraperitoneally at the start of study). Blood samples were collected for biochemical and hormonal assays. Testicular tissues were harvested for sperm analysis and histological studies. Data was presented as mean±standard deviation (SD) and significant difference level was p<0.05. Repeated oral administration of AT after exposure to PB caused significant increase in serum levels of catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC), follicle stimulating hormone (FSH), leutenizing hormone (LH), testosterone, 17β-hydroxysteroid dehydrogenase (17HSD) and sperm parameters compared to PB only treated group. Further, significant reduction in serum levels of ROS, RNS, and abnormal sperm parameters were observed in the co-treated group compared to PB only treated group. Testicular histoarchitecture also revealed an improvement of the deleterious effect of PB due to the AT treatment. Hence, AT ameliorates PB-induced reproductive impairments in rats.
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