Isolation of rhizobacteria belonging to Bacillus spp. from different Agro-climatic zones of India and their evaluation as plant stimulants
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Abstract
The plant-growth promoting potency of 161 native Bacillus spp. isolates was evaluated at Prof. TNA Innovation Center, Hyderabad, India during 2020-22. In preliminary screening, 19 isolates exhibited superior plant-growth promoting activities. High activities of amylase (>1.3 cm; 8 isolates), protease (>1.2 cm; 9 isolates), cellulase (>1.2 cm; 15 isolates), ammonia (9 isolates), IAA (>10 µg/mL; 9 isolates), and exopolysaccharide production (>0.05 w/v; 7 isolates) were recorded among these isolates. Nutrient solubilization viz., phosphate (10 isolates ranging, 0.30-57.3 μg/mL), zinc (8 isolates ranging, 0.07-0.30 μg/mL), potash (9 isolates ranging, 5.5-45.7 μg/mL), silica (6 isolates ranging, 0.10-0.40 μg/mL) was recorded. None of the isolates showed sulphur mobilization activity. In vitro pot studies using Vigna radiata as a test plant recorded maximum shoot length (19.47 cm), root length (9.42 cm), and plant dry mass (2.11 gm) when the seeds were treated with isolate B7. The seed germination was high in the B7, B79 and B88 inoculation treatments. Based on the in vitro and in vivo results, 5 of the best performing Bacillus isolates were shortlisted and evaluated for seed and seedling vigour in Sorghum biocolor and V. radiata, and field bio-efficacy studies using Capsicum annuum. Results demonstrated significantly high seed and seedling vigor, and yield (7300 kg/ha) when B7 isolate was used as seed dress and soil application. The isolated B7 was identified as Aneurinibacillus migulanus with accession no: NR_112214.1. Efforts are being made to carry out multi-location bio-efficacy studies using the A. migulanus on C. annuum during the rainy season 2022.
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