Optimization of Arthrospira platensis growth using organic culture medium

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Aporn Bualuang
Bongkoj Boonburapong
Surasak Laloknam

Abstract

In this study, an organic culture medium for Arthrospira platensis (Spirulina) was created using sugarcane molasses (SGCM), soybean meal (SBM), and small fish bone meal (SFBM) as substitutions for macronutrients and micronutrients in Zarrouk’s medium. Cell growth of A. platensis was monitored using spectrophotometric analysis. The growth patterns of A. platensis under normal and lack of carbon, nitrogen, and micronutrient conditions showed that the optimal growth of a lack of carbon, nitrogen, and micronutrient was decreased by about 50% compared to normal conditions. A singular material was designed to substitute macronutrients and micronutrients in Zarrouk’s medium. In the SGCM medium, the total reducing sugars were varied by concentration in the range of 0 to 3.0% (w/v), while the total protein in the SBM medium was similar. The percentage of dried SFBM in the SFBM medium was also in the concentration range of 0 to 1.0 % (w/v). The results showed that the optimum concentrations of the SGCM, SBM and SFBM media were 0.2% (w/v), 0.5% (w/v) and 0.1% (w/v), respectively. An organic combination medium was designed using optimal concentrations of SGCM, SBM, and SFBM. For a period of 30 days, cell growth of A. platensis was studied and compared to Zarrouk's medium. The wet weights of A. platensis in the combination medium and Zarrouk's medium were 31.25 g and 33.46 g, respectively. The findings indicated that the combination-medium composed of organic substance medium could be used successfully as a culture medium for A. platensis within organic system.

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How to Cite
Bualuang, A., Boonburapong, B., & Laloknam, S. (2022). Optimization of Arthrospira platensis growth using organic culture medium. Asia-Pacific Journal of Science and Technology, 27(05), APST–27. https://doi.org/10.14456/apst.2022.68
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ICoFAB 2021 Articles

References

Grosshagauer S, Kraemer K, Somoza V. The true value of Spirulina. J Agric Food Chem. 2020;68(14): 4109-4115.

Molino A, Iovine A, Casella P, Mehariya S, Chianese S, Cerbone A, et al. Microalgae characterization for consolidated and new application in human food, animal feed, and nutraceuticals. Int J Environ Res Public Health. 2018;15(11):2436.

Camacho F, Macedo A, Malcata F. Potential industrial applications and commercialization of microalgae in the functional food and feed industries: a short review. Mar Drugs. 2019;17(6):312.

Gómez-Zorita S, Trepiana J, González-Arceo M, Aguirre L, Milton-Laskibar I, González M, et al. Anti-obesity effects of microalgae. Int J Mol Sci. 2020;21(1):41.

Mitra M, Mishra S. Multiproduct biorefinery from Arthrospira spp. towards zero waste: current status and future trends. Bioresour Technol. 2019;291:121928.

Rezaei R, Akbulut A, Sanin SL. Effect of algae acclimation to the wastewater medium on the growth kinetics and nutrient removal capacity. Environ Monit Assess. 2019;191(11):1-10.

Zhan J, Rong J, Wang Q. Mixotrophic cultivation, a preferable microalgae cultivation mode for biomass/bioenergy production, and bioremediation, advances, and prospect. Int J Hydrog Energy. 2017;42(12):8505-8517.

Gould D, Compagnoni A, Lembo G. Organic aquaculture: principles, standards, and certification. In: Lembo G, Mente E, editors. Organic Aquaculture. Cham: Springer; 2019. p. 1-22.

Gambelli D, Naspetti S, Zander K, Zanoli R. Organic aquaculture: economic, market and consumer aspects. In: Lembo G, Mente E, editors. Organic Aquaculture. Cham: Springer; 2019. p. 41-63.

Sukumaran P, Nulit R, Halimoon N, Simoh S, Omar H, Ismail A. Formulation of cost-effective medium using urea as a nitrogen source for Arthrospira platensis Cultivation under real environment. Annu Res Rev Biol. 2018;22(2):1-12.

Palmonari A, Cavallini D, Sniffen C, Fernandes L, Holder P, Fagioli L, et al. Characterization of molasses chemical composition. J Dairy Sci. 2020;103(7):6244-6249.

Edwards CH, Rossi M, Corpe CP, Butterworth PJ, Ellis PR. The role of sugars and sweeteners in food, diet, and health: alternatives for the future. Trends Food Sci Technol. 2016;56:158-166.

Lagos L, Stein HH. Chemical composition and amino acid digestibility of soybean meal produced in the United States, China, Argentina, Brazil, or India. J Anim Sci. 2017;95(4):1626-1636.

García-Rebollar P, Cámara L, Lázaro R, Dapoza C, Pérez-Maldonado R, Mateos G. Influence of the origin of the beans on the chemical composition and nutritive value of commercial soybean meals. Anim Feed Sci. 2016;221:245-261.

Chakraborty P, Mallik A, Sarang N, Lingam S. A review on alternative plant protein sources available for future sustainable aqua feed production. Int J Chem Stud. 2019;7(3):1399-1404.

Cashion T, Le Manach F, Zeller D, Pauly D. Most fish destined for fishmeal production are food-grade fish. Fish Fish. 2017;18(5):837-844.

Setyahadi S. Utilization of fish waste for the making of fish sauce. In: Kim SK, editor. Seafood processing by-products. New York: Springer; 2014. p. 171-181.

Noordin NM, Kader MA, Morni MM, Raes AMA, Daud MSM, Khir RM, et al. Application of fish bone meal from byproducts of fish processing industry in diets of juvenile striped catfish, Pangasianodon hypophthalmus. AACL Bioflux 2017;10(6):1395-1403.

Duangsri C, Mudtham NA, Incharoensakdi A, Raksajit W. Enhanced polyhydroxybutyrate (PHB) accumulation in heterotrophically grown Arthrospira platensis under nitrogen deprivation. J Appl Phycol. 2020;32(6):3645-3654.

Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31(3):426-428.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254.

Raoof B, Kaushik B, Prasanna R. Formulation of a low-cost medium for mass production of Spirulina. Biomass Bioenergy. 2006;30(6):537-542.

El-Shouny W, Sharaf M, Abomohra A, Abo-Eleneen M. Production enhancement of some valuable compounds of Arthrospira platensis. J Bas Environ Sci. 2015;2:74-83.

Li X, Li W, Zhai J, Wei H, Wang Q. Effect of ammonium nitrogen on microalgal growth, biochemical composition and photosynthetic performance in mixotrophic cultivation. Bioresour Technol. 2019;273:368-376.

Rodrigues MS, Ferreira-Camargo LS, Converti A, Sato S, Carvalho JCM. Ammonium feeding and pH influencing Arthrospira platensis growth, composition, and C- and N-uptake. Chem Eng Technol. 2016;39(9):1700-1706.

Grobbelaar JU. Inorganic algal nutrition. In: Richmond A, Hu Q, editors. Handbook of microalgal culture: applied phycology and biotechnology. 2nd ed. Chichester: John Wiley&Sons; 2013. p. 123-133.

Markou G. Alteration of the biomass composition of Arthrospira (Spirulina) platensis under various amounts of limited phosphorus. Bioresour Technol. 2012;116:533-535.

Andrade MR, Costa JA. Mixotrophic cultivation of microalga Spirulina platensis using molasses as organic substrate. Aquac. 2007;264(1-4):130-134.

Budiyono, Syaichurrozi I, Sumardiono S, Budi Sasongko S. Production of Spirulina platensis biomass using digested vinasse as cultivation medium. Trends Appl Sci Res. 2014;9(2):93-102.

Dineshkumar R, Umamageswari P, Jayasingam P, Sampathkumar P. Enhance the growth of Spirulina platensis using molasses as organic additives. World J Pharm Res. 2015;4(6):1057-1066.

Yeesang C, Cheirsilp B. Low-cost production of green microalga Botryococcus braunii biomass with high lipid content through mixotrophic and photoautotrophic cultivation. Appl Biochem Biotechnol. 2014;174(1):116-129.

Li S, Zhu D, Li K, Yang Y, Lei Z, Zhang Z. Soybean curd residue: composition, utilization, and related limiting factors. Int Sch Res Notices. 2013;2013:1-8.

Shanthi G, Premalatha M, Anantharaman N. Effects of L-amino acids as organic nitrogen source on the growth rate, biochemical composition and polyphenol content of Spirulina platensis. Algal Res. 2018;35:471-478.

El-Sayed AFM. Chapter 7 Nutrition and feeding. In: El-Sayed AFM, editor. Tilapia culture. 2nd ed. London: Academic Press; 2020. p. 135-172.

Barlow SM. Fish meal. In: Caballero B, editor. Encyclopedia of food sciences and nutrition. 2nd ed. Oxford: Academic Press; 2003. p. 2486-2491.

Stunda-Zujeva A. Review of Spirulina/Arthrospira growth medium modifications. Key Eng Mater. 2020;850:153-158.

Bandara J, Arunakumara K. Development of low-cost growing media for Spirulina using alternative carbon sources. Vidyodaya J Sci. 2020;23(1):41-47.

Madkour FF, Kamil AEW, Nasr HS. Production and nutritive value of Spirulina platensis in reduced cost media. Egypt J Aquat Res. 2012;38(1):51-57.

Pumas P, Pumas C. Cultivation of Arthrospira (Spirulina) platensis using a low-cost medium supplemented with lac wastewater. Chiang Mai J Sci. 2016;43(5):1037-1047.