Asymbiotic germination of Habenaria rhodocheila Hance on different culture media and impact of plant growth regulators

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Published: Sep 5, 2022
DOI: https://doi.org/10.14456/apst.2022.82
Keywords:
Conservation Cytokinins Terrestrial orchid Seed germination Seedling development

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Theera Thummavongsa
School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand
Santi Watthana
School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand
Chuthapond Musimun
School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand
Nooduan Muangsan
School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand

Abstract

Habenaria rhodocheila Hance is a very popular terrestrial orchid due to the diversity of its floral color. Since its seed germination is low, modification of the culture media by adding hormones could enhance the germination rate. This study focused on the asymbiotic seed germination of H. rhodocheila’s orange and pink forms. Four media (Vacin and Went (VW), ½ VW, Murashige and Skoog (MS), and ½ MS), and three hormones (6-benzylaminopurine (BAP), gibberellic acid (GA), and thidiazuron (TDZ) at 1, 3, 5 mg/L concentrations were investigated for seed germination and protocorm formation for 16 weeks. The results revealed that seeds of both orange and pink forms germinated within four weeks after inoculation and the pink form seeds had a higher seed germination percentage than the orange form seeds in all tested media. Both orange and pink forms had the highest seed germination percentages on ½VW with 15.78±3.82% and 27.92±3.79%, respectively. Protocorm development of the pink form reached stage 5 in all tested media, but for the orange form it was observed only in VW and 1/2 VW. The addition of 1 mg/L BAP in ½VW media enhanced seed germination and facilitated the advanced prototocorm stage when compared with the control, while GA and TDZ showed an inhibitory effect on germination and protocorm formation. The results indicate that asymbiotic seed germination on ½ VW when added 1 mg/L BAP is an effective condition for this orchid species. This result may be applied for commercial production of this species and other terrestrial orchids.

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How to Cite
Thummavongsa, T., Watthana, S., Musimun, C., & Muangsan, N. (2022). Asymbiotic germination of Habenaria rhodocheila Hance on different culture media and impact of plant growth regulators . Asia-Pacific Journal of Science and Technology, 27(05), APST–27. https://doi.org/10.14456/apst.2022.82
Issue
Vol. 27 No. 05 (2022): SEP-OCT
Section
Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Pridgeon AM, Cribb PJ, Chase MW, Rasmussen FN, editors. Genera Orchidacearum: orchidoideae, part 1. 2 vols. 1st ed. Oxford: Oxford University Press; 2001.

Kurzweil H. The genus Habenaria (Orchidaceae) in Thailand. TFB. 2009;37(Special Issue):7-105.

Teoh ES. Medicinal orchids of Asia. 1st ed. Cham: Springer International Publishing AG; 2016.

Sinumporn P, Kawasaki TN, Fukai S. Development of interspecific hybrids between Habenaria radiata and Habenaria rhodocheila complex. Adv Hort Sci. 2020;34(1):3-10.

Swarts ND, Dixon KW. Conservation methods for terrestrial orchids. 1st ed. Florida: J Ross publishing; 2017.

Stewart SL, Zettler LW. Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H. quinqueseta, H. macroceratitis) from Florida. Aquat Bot. 2020;72(1):25-35.

Hossain MM, Kant R, Pham V, Winarto B, Zeng S, Silva JT. The application of biotechnology to orchids. Crit Rev Plant Sci. 2013;32(2):69-139.

Piyatrakul P, Apavatjrut P. Effect of pod age on seed germination of Habenaria rhodocheila Hance in aseptic conditions. J Agric. 2004;20(3):230-234.

Diengdoh R, Kumaria S, Tandon P, Das M. Asymbiotic germination and seed storage of Paphiopedilum insigne, an endangered lady's slipper orchid. S Afr J Bot. 2017;112:215-224.

Stewart S, Kane M. Asymbiotic seed germination and In vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Org. 2006;86(2):147-158.

Gayatri MC, Kavyashree R, Usha SR, Dhananjaya SP. In vitro seed germination of Pecteilis gigantea: a rare and endangered orchid from Western ghats. Plant Cell Biotechnol Mol Biol. 2006;7(3):191-194.

Dowling N, Jusaitis M. Asymbiotic In vitro germination and seed quality assessment of Australian terrestrial orchids. Aust J Bot. 2012; 60:592-601.

Sotthikul C, Kaewpoowat C, Saimoon N. In vitro propagation of Habenaria hybrids. Acta Hortic. 2017;1155:293-300.

Barrientos B, Fang JY. Influence of photoperiod and culture medium on the speed of asymbiotic seed germination and seedling development in Spathoglottis plicata. HortScience. 2019;54(9):1570-1575.

Manrique JP, Lizarazo CF, Suarez SA. Evaluation of the effect of three growth regulators in the germination of Comparettia falcata seeds under In vitro conditions. In Vitro Cell Dev Biol Plant. 2005;41(6):838-843.

Kim DH, Kang KW, Enkhtaivan G, Jan U, Sivanesan I. Impact of activated charcoal, culture medium strength and thidiazuron on non-symbiotic In vitro seed germination of Pecteilis radiata (Thunb.) Raf. S Afr J Bot. 2019;124:144-150.

Giri L, Jugran A, Rawat S, Dhyani P, Andola H, Bhatt ID, et al. In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: an important Astavarga plant. Acta Physiol Plant. 2012;34(3):869-875.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473-497.

Vacin EF, Went FW. Some pH changes in nutrient solutions. Botanical Gazette. 1949; 110(4):605-613.

Papenfus HB, Naidoo D, Posta M, Finnie JF, Staden JV. The effects of smoke derivatives on in vitro seed germination and development of the leopard orchid Ansellia africana. Plant Biol. 2015;18(2):289-294.

Malmgren S. Orchid propagation: theory and practice. In: Allen C, editor. North American native terrestrial orchids: propagation and production. 1996 Mar 16-17; Washington, United States. Philadelphia: Biodiversity Heritage Library;1996. p. 63-71.

Saikum C, Kunasakdakul K, Potapohn N. Crossability and In vitro seed germination of some terrestrial orchid species of genus Habenaria and Pecteilis. J Agric. 2020;36(1):47-58.

Gayatri MC, Kavyashree R, Usha SR, Dhananjaya SP. In vitro seed germination of Pecteilis gigantea: a rare and endangered orchid from Western ghats. Plant Cell Biotechnol Mol Biol. 2006;7(3):191-194.

Godo T, Komori M, Nakaoki E, Yukawa T, Miyoshi K. Germination of mature seeds of Calanthe tricarinata Lindl., an endangered terrestrial orchid, by asymbiotic culture in vitro. In Vitro Cell Dev Biol Plant. 2010;46(3):323-328.

Bektaş E, Cuce M, Sokmen A. In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey. Turk J Bot. 2013;37(1):336-342.

Bektaş E, Sokmen A. In vitro seed germination, plantlet growth, tuberization, and synthetic seed production of Serapias vomeracea (Burm.f.) Briq. Turk J Bot. 2016;40(6):584-594.

Cheruvathur MA, Abraham J, Mani B, Thomas D. Adventitious shoot induction from cultured internodal explants of Malaxis acuminata D. Don, a valuable terrestrial medicinal orchid. Plant Cell Tiss Org. 2010;101(2):163-170.

Thomas D, Michael A. High-frequency plantlet regeneration and multiple shoot induction from cultured immature seeds of Rhynchostylis retusa Blume., an exquisite orchid. Plant Biotechnol Rep. 2007;1(4):243-249.

Arditti J, Ernst R. Micropropagation of orchids. 1st ed. New York: John Wiley & Sons, Inc.; 1993.

Rasmussen N. Terrestrial orchids, from seed to mycotrophic plant. 1st ed. Cambridge: Cambridge University Press; 1995.