Asymbiotic germination of Habenaria rhodocheila Hance on different culture media and impact of plant growth regulators

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Theera Thummavongsa
Santi Watthana
Chuthapond Musimun
Nooduan Muangsan


Habenaria rhodocheila Hance is a very popular terrestrial orchid due to the diversity of its floral color. Since its seed germination is low, modification of the culture media by adding hormones could enhance the germination rate. This study focused on the asymbiotic seed germination of H. rhodocheila’s orange and pink forms. Four media (Vacin and Went (VW), ½ VW, Murashige and Skoog (MS), and ½ MS), and three hormones (6-benzylaminopurine (BAP), gibberellic acid (GA), and thidiazuron (TDZ) at 1, 3, 5 mg/L concentrations were investigated for seed germination and protocorm formation for 16 weeks. The results revealed that seeds of both orange and pink forms germinated within four weeks after inoculation and the pink form seeds had a higher seed germination percentage than the orange form seeds in all tested media. Both orange and pink forms had the highest seed germination percentages on ½VW with 15.78±3.82% and 27.92±3.79%, respectively. Protocorm development of the pink form reached stage 5 in all tested media, but for the orange form it was observed only in VW and 1/2 VW. The addition of 1 mg/L BAP in ½VW media enhanced seed germination and facilitated the advanced prototocorm stage when compared with the control, while GA and TDZ showed an inhibitory effect on germination and protocorm formation. The results indicate that asymbiotic seed germination on ½ VW when added 1 mg/L BAP is an effective condition for this orchid species. This result may be applied for commercial production of this species and other terrestrial orchids.


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Thummavongsa, T., Watthana, S., Musimun, C., & Muangsan, N. (2022). Asymbiotic germination of Habenaria rhodocheila Hance on different culture media and impact of plant growth regulators . Asia-Pacific Journal of Science and Technology, 27(05), APST–27.
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