Halotolerant rhizobacterial community on the growth of tomato plants under salinity condition
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Abstract
Bio-fertilizer is a product used for improving soil quality. Microorganisms subsisting in bio-fertilizer have the ability to cycle nutrients in soils leading to enhanced soil fertility. Thus, the aim of this study was to investigate the ability of salt tolerant rhizobacteria to promote the growth of tomato plants in pot experiments under salinity conditions. Also, microbial communities within the cultivated soil samples were investigated. Three isolates of salt tolerant rhizobacteria including Enterobacter aerogenes P8, Bacillus tequilensis N15 and Pseudomonas azotoformans I2.1 were isolated from rhizosphere soil of rice plant and cultivated on soil supplemented with various salinity conditions. All isolates were able to live at the surface area of the tomato roots. Their abilities to promote tomato growth under different salinity conditions were investigated. The results showed that the inoculation of rhizobacteria which immobilized on carriers enhanced tomato plants biomass under two salinity levels (0%, 0.25% NaCl). Moreover, plants of both treatments showed an increase in total nitrogen, available phosphate and exchangeable potassium when compared to other treatments. According to the profile of rhizosphere bacteria community obtained using denaturing gradient gel electrophoresis (DGGE) analysis, eight species of bacteria including Klebsiella aerogenes, Enterobacter sp., Caballeronia concitans , Raoultella planticola, Citrobacter freundii , Burkholderia thailandensis , Frateuria sp., and Fulvimonas sp. were identified. This is the first report to show a member of Fulvimonas sp. in the tomato rhizosphere. These findings indicate that all of these bacteria might play a role in enhancing the growth of tomato plants.
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