Validation and application of HPLC method for determination of cordycepin and adenosine in dietary supplements
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Abstract
A simple method based on High Performance Liquid Chromatography (HPLC)-UV was developed in this study for determining the existence of cordycepin and adenosine in Cordyceps and its products. Chromatographic separation was carried out using a Kinetex® C18 column with a mobile phase of water and acetonitrile (95:5, v/v), a flow rate of 1.0 mL/min, and wavelengths of ultraviolet detection set at 260 nm. The calibration curve was linear over the range of 0.05–20 and 0.02–20 mg/mL for cordycepin and adenosine, respectively, and the limit of detection was 0.005 mg/mL for both compounds. The method was validated with excellent specificity, accuracy, precision, and recovery. The assay has been applied successfully to a quality control study on commercial products containing Cordyceps in the Thai market such as dried cultured materials and pharmaceutical formulations.
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