Validation and application of HPLC method for determination of cordycepin and adenosine in dietary supplements

Main Article Content

Naruemon Chernthong
Suphisara Noisakul
Aurasorn Saraphanchotiwitthaya
Pattana Sripalakit

Abstract

A simple method based on High Performance Liquid Chromatography (HPLC)-UV was developed in this study for determining the existence of cordycepin and adenosine in Cordyceps and its products. Chromatographic separation was carried out using a Kinetex® C18 column with a mobile phase of water and acetonitrile (95:5, v/v), a flow rate of 1.0 mL/min, and wavelengths of ultraviolet detection set at 260 nm. The calibration curve was linear over the range of 0.05–20 and 0.02–20 mg/mL for cordycepin and adenosine, respectively, and the limit of detection was 0.005 mg/mL for both compounds. The method was validated with excellent specificity, accuracy, precision, and recovery. The assay has been applied successfully to a quality control study on commercial products containing Cordyceps in the Thai market such as dried cultured materials and pharmaceutical formulations.

Article Details

How to Cite
Chernthong, N., Noisakul, S., Saraphanchotiwitthaya, A., & Sripalakit, P. (2022). Validation and application of HPLC method for determination of cordycepin and adenosine in dietary supplements. Asia-Pacific Journal of Science and Technology, 27(06), APST–27. https://doi.org/10.14456/apst.2022.99
Section
Research Articles

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