Effect of thyme oil on mechanical and antimicrobial potential of solvent casted poly (3-hydroxybutyrate)
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Abstract
Poly(3-hydroxybutyrate) (PHB) is a biopolymer that substitutes petroleum-based conventional plastics in the packaging sector. Production of PHB is presently limited due to its higher cost of production. Therefore, this work has been designed to synthesize PHB by utilizing orange peel as a cheap carbon source. Pseudomonas putida has been used to accumulate PHB in the medium with orange peel hydrolysate. This strain has produced 5.2±0.03 g/L of PHB in orange peel hydrolysate medium under 72 h of incubation. The organism was able to synthesize 55% PHB of the cell biomass. Extracted PHB was confirmed by Nuclear Magnetic Resonance (NMR). Solvent casted films of extracted (neat) PHB and thyme oil (TO) (30%) incorporated PHB were prepared. Both the films were tested mechanically and microbiologically. The incorporation of TO reduces the tensile strength up to 22 % more than that of pure or neat PHB film, but improves the antimicrobial potential. TO incorporated PHB film was resistant against Bacillus subtilis (Gram positive), Candida albicans (fungal strain) and Escherichia coli (Gram negative). These findings suggest that TO incorporated PHB films could be used to develop eco-friendly active packaging products.
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