Anti-urolithiasis potential of Aerva lanata metabolites investigated in synthetic urine and cell-free in vitro assays
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Abstract
Urolithiasis commonly referred to as kidney stone disease, is a painful urologic condition that can cause rapid deterioration of renal function. Though there are several options for anti-urolithiasis treatment there is a need to avoid stone recurrence, maintain renal functions and to get a treatment which is economical. In this study, we investigated the anti-urolithiasis potential of whole plant extracts of the medicinal herb Aerva lanata. We identified the potential phytochemicals in A. lanata with a majority of α-Tocopherol, γ-Sitosterol and campesterol being identified using gas chromatography-mass spectrometry (GC-MS). In the in-vitro study using nucleation, aggregation and oxalate depletion assays, the inhibition efficiency of the plant extract at different stages of stone development was tested, where the experiments were performed in triple runs. The results showed a maximum of average crystal area reduction of up to 88.73% and a 52.36% stone formation inhibition at a concentration of 3mg/mL during a 30 min incubation period. This pilot study thus highlights the anti-urolithiatic efficiency of A. lanata metabolites.
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