The effect of substrates on the production of tropical mushroom Macrocybe crassa
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Abstract
The wild Thai isolate strain, the Department of Agriculture (DOA-10), of the mushroom, Macrocybe crassa, was grown on seven individual substrate formulas in order to measure mycelium development, productivity, morphological characteristics, and the nutritive value of the fruit-body. The most suitable substrate for cultivating this mushroom was spent mushroom, substrate-based substrate (T6), which consisted of spent mushroom substrate from Pleurotus eryngii cultivation, fine rice bran, MgSO4H2O, CaO, and fine corn seeds in a ratio of 100:3:0.2:1:0.5 (w/w). Mycelium growth rate and the number of fruit-bodies were higher when grown on this substrate formula compared to others. Moreover, this strain showed the fewest days to complete spawn running and primordial initiation. Additionally, its yield was highest at 237.21 g/0.6 kg of substrate, and the biological efficiency was highest at 65.89%. Mushroom morphology showed that the largest pileus and stipe diameters were found to be 117.00 and 37.20 mm, respectively, on spent mushroom substrate-based substrate (T6). Dry M. crassa strain DOA-10 grown on this substrate contained carbohydrates and crude proteins at 74.75% and 30.89%, respectively, which were higher than the other formulas. These results confirmed that spent mushroom substrate-based substrate (T6) is of high quality and could be a promising substrate for commercial cultivation of M. crassa native strain DOA-10.
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