Synthesis, characterization and antimicrobial activity of zinc oxide nanoparticles against Escherichia coli and Salmonella enterica-water borne pathogens

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Published: Apr 22, 2024
DOI: https://doi.org/10.14456/apst.2024.40
Keywords:
Antimicrobial Nanoparticles Pathogen ZnO NPs

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Pushpa B. Negi
Department of Chemistry, Graphic Era Hill University, Bhimtal Campus, Uttarakhand, India
Anita Rana
Rajendra Singh Nanoscience and Nanotechnology Centre, Department of Chemistry, Kumaun University, Nainital, D.S.B. Campus, Uttarakhand, India
Naveen C. Joshi
Division of Research and Innovation, Uttaranchal University, Uttarakhand, India
Abhilasha Mishra
Department of Chemistry, Graphic Era deemed to be University, Uttarakhand, India
Manoj C. Lohani
Department of School Computing, Graphic Era Hill University, Bhimtal Campus, Uttarakhand, India
Sandeep K. Sunori
Department of Electronics and Communication Engineering, Graphic Era Hill University, Bhimtal Campus, Uttarakhand, India

Abstract

Waterborne pathogens viz. Escherichia coli (E. coli) and Salmonella enterica (S. enterica) and their associated diseases are key public health threat worldwide, causing significant morbidity and mortality thereby responsible for high public health expenditure and consequent economic burden. Therefore, the research aimed to explore the facile synthesis of zinc oxide nanoparticles (ZnO NPs) using (CH3COO)2Zn.2H2O as precursor and further evaluation of their antimicrobial activity. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), Energy Dispersive X-Ray Analysis (EDX), particle size analyser (PSA) and thermogravimetric analysis (TGA) have been used to characterize the synthesized NPs. The binding of Zn by Zn-O stretching was validated by FTIR spectrum whereas the identity of the crystalline ZnO wurtzite-type material was established by XRD. SEM imaging revealed the sphere/petal shaped agglomerated particles. Furthermore, average particle size of NPs was 702.9 nm, measured by PSA. Wall zeta potential value of synthesized particles was -16.44 reflecting the agglomerating nature of the material. TGA analysis showed that the material was highly thermostable and 88% remained stable at 760°C. The qualitative well diffusion test conducted for evaluating the antimicrobial activity of synthesized material resulted in noticeable inhibitory activity against E. coli (20±0.2) and S. enterica (18±0.1). The sensitivity exhibited by both the test microbes was high @15µg of ZnO NPs whereas at lower concentrations no sensitivity was reported. Thus, the synthesized ZnO NPs played a significant role in antimicrobial activity and could be an alternative antibacterial agent in the treatment of waterborne infections.

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Negi, P. B., Rana, A., Joshi, N. C. ., Mishra, A. . ., Lohani, M., & Sunori , S. K. . (2024). Synthesis, characterization and antimicrobial activity of zinc oxide nanoparticles against Escherichia coli and Salmonella enterica-water borne pathogens. Asia-Pacific Journal of Science and Technology, 29(03), APST–29. https://doi.org/10.14456/apst.2024.40
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Vol. 29 No. 03 (2024): MAY-JUNE
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Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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