Community of endophytic bacteria colonized in Jerusalem artichoke observed under molecular technique
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Abstract
Plant associated bacteria, named endophytic bacteria, colonize inside plant organs and mostly interact via plant growth promotion. The location of bacteria inside plants is different depending on bacterial species; likely, the bacterial diversity is different depending on plant species and stress environments. The bacterial community was determined in parts of Jerusalem artichoke which grew under well watering and deficient water conditions with inoculation of endophytic bacteria, Rossellomorea aquimaris 3.13, Micrococcus luteus 4.43 and Bacillus velezensis 5.18. The techniques used to determine community and colonization were denaturing gradient gel electrophoresis and transmission electron microscopy, respectively. The root showed the highest bacterial diversity, particularly under drought condition. The endophytic bacteria inoculated in the plants had negative effect on bacterial community. In contrast, the drought stress did have an effect on bacterial community. The majority of endophytic bacteria were members of gamma-Proteobacteria and Firmicutes (Bacillus spp.). The colonization of three endophytic bacteria was observed in the xylem vessel of stem and root of Jerusalem artichoke. Results showed that the endophytic bacteria were localized where they were isolated, leading to an understand of the relationship between endophytic bacteria and plant host.
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