TLR4, BTN1A1, and Pit-1 polymorphisms and their associations with the estimated breeding values of growth traits of Brahman cattle in Thailand
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Abstract
The molecular analysis of genes is considered an acceptable tool for accelerating the genetic improvement of production performance in cattle. Single nucleotide polymorphisms (SNPs) in Toll-like receptor 4 (TLR4) and Butyrophilin subfamily 1 member A1 (BTN1A1) genes are related to immunity and may affect all aspects of cattle production. Pituitary-specific positive transcription factor 1 (Pit-1) is related to expression of the growth hormone-releasing factor gene. There were used in this study to examine the associations with estimated breeding values (EBVs) of growth traits [birth weight (BW), weaning weight (WW), and yearling weight (YW)] of Brahman cattle. Two hundred sixty-five cattle blood samples were obtained for DNA extraction, and gene polymorphisms were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The TLR4 gene was associated with the EBV of WW, which was significantly higher with the AB genotype than with the AA genotype (p ≤0.05) in cattle. This gene was associated with the EBV of YW, which was significantly higher in the cattle with the AB genotype than in those with the AA genotype (p ≤0.05). Cattle with the genotypes AA and AB had a higher (p ≤0.05) EBV of WW than cattle with the BB genotype of the BTN1A1 gene, which was also significant. The Pit-1 gene was associated with the EBV of BW, which was significantly lower in cattle with the genotype BB than in those with the genotype AA (p ≤0.05). The possibility of selecting growth traits using these three genetic markers is therefore interesting.
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