Screening of Lipolytic producing microbes from microbial consortium for treatment of palm oil contaminated wastewater
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Abstract
FOGs (fat, oil, and grease) pollution in domestic wastewaters has been a significant environmental issue worldwide. This study was designed to determine the potential of bacterial strains isolated from wastewater microbial consortiums to biodegrade the FOGs. The isolated bacteria were characterized by standard Gram staining method and lipid degradation assays. Gram staining showed that four of the selected isolates, (M9P8, MRS-1A.3W, MRS-1B-1W, and MRS-1B-1.C) were Gram-positive, rod-shaped bacteria. One isolate PCA-3A.1.Y, was a Gram-positive coccus. All five bacteria displayed lipase activity as demonstrated by tributyrin hydrolysis when tested using the drop plate method on tributyrin agar. Lipase activity was found to be highest in MRS-1B-1. W strain, at 5.89±2.16 U lipase/mg protein. Further analysis was conducted to determine the potential for palm oil reduction. The results showed that, in 0.5 Plate Count Broth (PCB) with 1% (v/v) palm oil, the five bacterial isolates had a 7.29% to 32.37% reduction of palm oil. It is concluded that this approach has resulted in collection of promising bacterial isolates that may be useful for direct applications in biodegradation of lipid-rich wastewaters.
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