A portable RGB color sensor for detecting free fatty acid (FFA) in palm oil samples

Main Article Content

vakin Nunumvong
Kosin Teeparuksapun
Nicha Prasongchan
Apichai Phonchai

Abstract

A colorimetric sensor based on red, green, blue (RGB) measurement using an OV2640 camera module and ESP32 microcontroller was developed for measuring free fatty acid (FFA) in crude palm oil samples. The proposed method is based on measuring the color of liquid samples and convert it to RGB and Grayscale intensity. The RGB sensor’s performance was evaluated for application in analysing FFA in crude palm oil samples. The FFA analysis was based on the application of the limiting reagent concept in an acid-base (palmitic acid-Na2CO3) reaction with phenolphthalein as an indicator. The RGB sensor could detect FFA with a linear relationship in the range of 0.0 - 0.1 % w/w and the linear regression equation was y = 1,125x - 2.8056, r = 0.9923, with limit of detection (LOD) and limit of quantification (LOQ) having values of 0.017 and 0.057 % w/w, respectively. The analysis of FFA in crude palm oil samples showed good agreement with results obtained from the proposed RGB sensor and the titration method (AOCS; Ca5a-40). This simple, rapid, and economical analysis method for assessing FFA holds promise for application in the quality control of crude palm oil samples.

Article Details

How to Cite
Nunumvong, vakin, Teeparuksapun, K., Prasongchan, N., & Phonchai, A. (2024). A portable RGB color sensor for detecting free fatty acid (FFA) in palm oil samples. Asia-Pacific Journal of Science and Technology, 29(06), APST–29. https://doi.org/10.14456/apst.2024.92
Section
Research Articles

References

1 Saad B, Ling CW, Jab MdS, Lim BP, Ali ASM, Wai WT, Saleh MI. Determination of free fatty acids in palm oil samples using non-aqueous flow injection titrimetric method. Food Chem. 2007;102(4):1407-1144.

2 Che Man YB, Moh MH, van de Voort FR. Determination of free fatty acids in crude palm oil and refined-bleached-deodorized palm olein using fourier transform infrared spectroscopy. J Amer Oil Chem Soc. 1999;76(4):485-490.

3 Medeiros LMS, Lima AF, Gonçalves MC, Godoy HT, Barbin DF. Portable near-infrared (NIR) spectrometer and chemometrics for rapid identification of butter cheese adulteration. Food Chem. 2023;425:136461.

4 Gan HL, Tan CP, Man YBC, NorAini I, Nazimah SAH. Monitoring the storage stability of RBD palm olein using the electronic nose. Food Chem. 2005; 89(2):271-282.

5 Azeman NH, Yusof NA, Othman AI. Detection of free fatty acid in crude palm oil. Asian J Chem. 2015;27(5):1569-1573.

6 Piriya VSA, Joseph P, Daniel SCGK, Lakshmanan S, Kinoshita T, Muthusamy S. Colorimetric sensors for rapid detection of various analytes. Mater Sci Eng C. 2017;78:1231-1245.

7 Chaisiwamongkhol K, Phonchai A, Pon-In S, Bunchuay T, Limbut W. A microplate spectrophotometric method for analysis of indole-3-carbinol in dietary supplements using p-dimethylaminocinnamaldehyde (DMACA) as a chromogenic reagent. Anal Methods. 2022;14(35):3366-3374.

8 Choodum A, Malathong K, NicDaeid N, Limsakul C, Wongniramaikul W. A cost-effective hydrogel test kit for pre and post blast trinitrotoluene. Forensic Sci Int. 2016;266:202-208.

9 Lantam A, Limbut W, Thiagchanya A, Phonchai A. A portable optical colorimetric sensor for the determination of promethazine in lean cocktail and pharmaceutical doses. Microchem J. 2020;159:105519.

10 Teeparuksapun T, Prasongchan N, Thawonsuwan A. Alpha-lipoic acid functionalized silver nanoparticles for colorimetric detection of copper ion. Anal Sci. 2019;35(4):371-377.

11 Balbach S, Jiang N, Moreddu R, Dong X, Kurz W, Wang C, Dong J, Yin Y, Butt H, Brischwein M, Hayden O, Jakobi M, Tasoglu S, Koch AW, Yetisen AK. Smartphone-based colorimetric detection system for portable health tracking. Anal Methods. 2021;13(38):4361-4369.

12 Alawsi T, Mattia GP, Al-Bawi Z, Beraldi R. Smartphone-based colorimetric sensor application for measuring biochemical material concentration. Sens Bio-Sens Res. 2021;32:100404.

13 Park H, Koh YG, Lee W. Smartphone-based colorimetric analysis of structural colors from pH-responsive photonic gel. Sens Actuators B Chem. 2021;345:130359.

14 Leal VG, Batista AD, da Silveira Petruci JF. 3D-printed and fully portable fluorescent-based platform for sulfide determination in waters combining vapor generation extraction and digital images treatment. Talanta. 2021;222:121558.

15 Pazzi BM, Pistoia D, Alberti G. RGB-detector: A smart, low-cost device for reading RGB indexes of microfluidic paper-based analytical devices. Micromachines. 2022;13(10):1585.

16 Minz PS, Saini CS. RGB camera-based image technique for color measurement of flavored milk. Measurement: Food. 2021;4:100012.

17 Ghosh A, Satvaya P, Kundu PK, Sarkar G. Calibration of RGB sensor for estimation of real-time correlated color temperature using machine learning regression techniques. Optik. 2022;258:168954.

18 Oliveira GDC, Machado CCS, Inácio DK, da Silveira Petruci JF, Silva SG. RGB color sensor for colorimetric determinations: Evaluation and quantitative analysis of colored liquid samples. Talanta. 2022;24:123244.

19 Nizam AFA, Azri MEM, Zahari MAKM, Mahmud MS. Application of infrared spectroscopic analysis for quantification of free fatty acid content at palm oil mills. IOP Conf Ser Mater Sci Eng. 2020;99(1):012067

20 Ho LS, Nair A, Mohd Yusof H, Kulaveerasingam H, Jangi, M. Morphometry of lipid bodies in embryo, kernel and mesocarp of oil palm: its relationship to yield. Am J Plant Sci. 2014;5:1163-1173.

21 Kasmin H, Lazim AM, Awang R. Effect of heat treatments on the yield, quality and storage stability of oil extract from palm fruits. Malays J Anal Sci. 2016;20(6):1373-1381.

22 Japir AAW, Salimon J, Derawi D, Bahadi M, Al-Shuja’a S, Yusop MR. Physicochemical characteristics of high free fatty acid crude palm oil. OCL. 2017;24(5):D506.

23 Kumar PKP, Krishna AGG. Physicochemical characteristics of commercial coconut oils produced in India. Grasas Aceites. 2015;66(1):e062.

24 Namwong P, Jarujamrus P, Amatatongchai M, and Chairam S. Fabricating simple wax screen-printing paper-based analytical devices to demonstrate the concept of limiting reagent in acid-base reactions. J Chem Educ. 2018;95(2):305-309.

25 Junaidah MJ., Norizzah AR, Zaliha O, Mohamad S. Optimisation of sterilisation process for oil palm fresh fruit bunch at different ripeness. Int Food Res J. 2015;22:275-282.

26 Ali FS, Shamsuddin R, Yunus R. Effect of chopping oil palm fruit spikelets on the free fatty acid content release rate and its mechanical properties. Int J Res Eng Technol. 2014,3(1):511-516.

27 Hadi S, Ahmad D, Akande FB. Determination of the bruise indexes of oil palm fruits. J Food Eng. 2009;95:322-326.