Exploration of Patchouli (Pogostemon cablin Benth.) Indigenous Rhizosphere Bacteria as Biofertilizer Candidates in Dissolving Phosphate and Producing Growth Regulator
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Abstract
Patchouli is a plant that has high economic value due to it is extensively application in many different industries. Despite its economic importance, the production of Patchouli has struggled to meet the demands of these industries. Addressing this challenge, one potential approach involves harnessing the capabilities of indigenous rhizosphere bacteria to enhance Patchouli growth. This study aims to investigate indigenous rhizosphere bacteria associated with Patchouli, focusing on their ability to solubilize phosphate and produce indole-3-acetic acid (IAA) and gibberellins. The research commenced with the isolation of microbes, which were subsequently evaluated for their phosphate-solubilizing and growth regulator-producing capacities. The results revealed the selection of ten isolates using Pikovskaya media, characterized by morphological variations, with 60% identified as gram-negative bacteria. Notably, three isolates (N1R1, N1R3, N1R7) exhibited superior phosphate-solubilizing abilities, with the highest average clear zone diameter recorded at 10.0 mm. Moreover, isolate N1R1 demonstrated the highest IAA production at 73.03 µg/ml, while isolate N1R7 exhibited the most substantial gibberellin (GA3) production at 98.18 µg/ml. Molecular identification indicated that N1R1 resembled Providencia rettgeri, N1R3 resembled Enterobacter sp., and N1R7 resembled Erwinia sp. In conclusion, three indigenous rhizosphere bacteria associated with Patchouli exhibit promising potential for biofertilizer development, given their proficiency in phosphate solubilization and growth regulator production.
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