Development of ISSR-Derived SCAR marker for rapid and accurate authentication of West Indian arrowroot (Maranta arundinacea L.)
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Abstract
Arrowroot, or West Indian arrowroot (Maranta arundinacea L.), native to the Antilles, Mexico, and other Central American countries, has the potential to become a flour source. Species are primarily identified based on morphological characteristics, which are influenced by the environmental factors. Currently, the most accurate authentication method is through DNA markers or molecular fingerprinting. Arrowroot accessions from various provinces were distinguished from their related species using Inter-Simple Sequence Repeat (ISSR)-derived Sequence-Characterized Amplified Region (SCAR) markers. DNA profiling was conducted based on the results of DNA amplification using ISSR primers. The polymorphism data identified a fragment, UBC825Ma-924, which was generated by primer UBC825 and was only present in arrowroot genotypes. The specific band was sequenced, and SCAR primers (SC-UBC825Ma-630) were designed and synthesized to amplify the 630 bp band. Using the primer pair and total DNAs of M. arundinacea and related species, diagnostic PCRs produced specific amplification only in M. arundinacea genotypes. This approach is quick, reliable, simple, viable for authentication, and helpful for cultivar development and conservation.
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