Microclonal success of avocado using different scions and clonal rootstock varieties
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Abstract
This study explored the microclonal compatibility of various avocado clonal rootstocks and scion varieties, assessing their influence on growth parameters such as leaf production, chlorophyll content, stem length, and stem diameter. The study employed a randomized complete block design (RCBD) with split plots, focusing on different clonal rootstock and scion combinations. The results demonstrated significant interactions between the clonal stock and scion varieties, influencing key growth metrics. 'Hass' scions microcloned onto 'Purple' and 'Evergreen' clonal rootstocks produced 5.60 leaves each, showing superior growth. In terms of leaf area, 'Hass' scions microcloned onto 'Purple' clonal rootstocks had the largest leaf area of 36.11 cm², significantly higher than other combinations. The chlorophyll content (Soil Plant Analysis Development [SPAD] values) was highest when 'Hass' scions were microcloned onto 'Purple' clonal rootstocks, yielding a 35.82 SPAD values. The highest stem length (6.02 cm) was observed in ‘Hass’ scion microcloned on ‘Evergreen’ clonal rootstock, while the biggest stem diameter (0.300 cm) was observed in ‘Evergreen’ scion microcloned onto ‘Purple’ clonal rootstock. These findings suggest that the pairing of 'Hass' microclone on 'Purple' clonal rootstock, can optimize early plant growth, leaf production, and plant health, and significantly impacts plant vigor. Further research may be conducted to monitor long-term plant health and refine microcloning techniques for improved avocado cultivation.
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References
Mossie, M., Genanew, T., Elias, A., & Chanie, T. Households’ food consumption status and asset accumulation in the context of export market orientation: A case of avocado producers in Ethiopia. J Agric Food Res. 2025;20:101774.
Pradeep, P, Nagaraju, V. Exotic fruits cultivation: Focus on avocado. Agric Food E-Newsletter. 2025;20:45-48.
Lutta A, Kehbila A, Sitati C, Sunguti EM, Suljada T, Osano P. Challenges and opportunities for upgrading the avocado value chain in East Africa. Stockholm Environ Ins. 2024;32:3-5.
Nyakang CO, Ebere R, Marete E, Arimi JM. Avocado production in Kenya in relation to the world, avocado by-products (seeds and peels) functionality and utilization in food products. Appl Food Res. 2023;3(1):100275.
Bugudole NF, Nungula EZ, Kilasi N, Nzogela, Y. Assessment of avocado grafting practices and propagules quality against minimum certification standards in Tanzania. Cogent Food Agric. 2025;11(1):101080.
Sarker BC, Gomasta J. Technique time and etiolation applications influencing the grafting success in avocado (Persea americana M.). Int J Hortic Sci Technol. 2024;11(2):147-162.
Feng M, Augstein F, Kareem A, Melnyk CW. Plant grafting: Molecular mechanisms and applications. Mol Plant. 2024;17(1):75–91.
Wakchaure GC, Kumar D, Kumar S, Gawhale BJ, Meena KK, Sawant CP, Singh DK, Paramasivam S K, Minhas PS. Grafting wild rootstocks as a climate-resilient strategy to enhance productivity, quality and tolerance in eggplant under variable water stress induced by deficit irrigation. Agric Water Manag. 2025;314:109492.
Tedesco S, Fevereiro P, Kragler F, Pina A. Plant grafting and graft incompatibility: A review from the grapevine perspective. Sci Hortic. 2022;299:111019.
Adhikari PB, Xu Q, Notaguchi M. Compatible graft establishment in fruit trees and its potential markers. Agronomy. 2022;12(8):1981.
Bashir A, Ganai NA, Qayoom S, Yousuf MW. Influence of clonal rootstocks on major morphological characteristics of some exotic apple cultivars in Northern Himalayas of Kashmir Valley. Biol Forum Int J. 2023;15(8):58-64.
Zhang H, Yao D, Zhang G, Ali HSM, Li X, Li Y, Liang T, Zhao F, Yu S, Yu K. Effect of grafted scion varieties on apple root growth, carbon and nitrogen metabolism and microbiome in roots and rhizosphere soil. Appl Soil Ecol. 2025;206:105841.
Department of Science and Technology - Philippine Atmospheric, Geophysical and Astronomical Services Administration DOST-PAGASA. 7th edition. Climatic data. Musuan, Maramag, Bukidnon, the Philippines. 2025.
Esgrina FJO. Tan RJ. Response of different avocado microclonal rootstock varieties to etiolation time. Nativa Sinop. 2024;12(2):274-284.
Esgrina FJO, Tan RJ. Growth vitamin hormone and shading intensity affect the rhizo-caulogenesis of etiolated avocado microclonal rootstocks. Asian J Agric. 2025;9:160–173.
Jayswal DK, Lal N. Rootstock and scion relationship in fruit crops. Agriallis. 2020;2(11):3-6.
Fallik E, Tuvia SA, Chalupowicz D, Sarid SP, Presman MZ. Relationships between rootstock-scion combinations and growing regions on watermelon fruit quality. Agronomy. 2019;9(9):536-539.
Rasool A, Mansoor S, Bhat KM, Hassan GI, Baba TR, Alyemeni MN, Alsahli AA, Serehy HAE, Paray BA, Ahmad P. Mechanisms underlying graft union formation and rootstock scion interaction in horticultural plants. Front Plant Sci. 2020;11:590847.
Martins T, Barros AN, Rosa E, Antunes L. Enhancing health benefits through chlorophylls and chlorophyll-rich agro-food: A comprehensive review. Molecules. 2023;28(14):5344.
Mandal R, Dutta, G. From photosynthesis to biosensing: Chlorophyll proves to be a versatile molecule. Sens Int. 2020;1:100058.
Yahia EM, López AC, Barrera, GM, Azpiri HS, Bolaños MQ. Photosynthesis. 7th ed. Postharvest physiology and biochemistry of fruits and vegetables. 2019:47-72.
Pâques LE. Rootstock effects on growth, reproduction, and wood properties in hybrid larch (Larix x eurolepis H.) and in some other Larix sp. grafting associations. Ann For Sci. 2025;82(9):10281.
Nivedha D, Rani MSA, Vethamoni PI, Lourdusamy DK, Vanitha K. Effect of different rootstocks and scions compatibility study on Rosa sp. Int Journal Environ Clim Change. 2023;13(10):2032–2039.
Valverdi NA, Cheng L, Kalcsits L. Apple scion and rootstock contribute to nutrient uptake and partitioning under different belowground environments. Agronomy. 2019;9(8):80415.
Lazare S, Haberman A, Yermiyahu U, Erel R, Simenski E, Dag A. Avocado rootstock influences scion leaf mineral content. Arch Agron Soil Sci. 2019;66(10):1399–1409.
Alfaro JM, Bermejo A, Navarro P, Quiñones A, Salvador A. Effect of rootstock on citrus fruit quality: A review. Food Rev Int. 2021;39(5):2835–2853.
Sallaku G, Rewald B, Sandén H, Balliu A. Scions impact biomass allocation and root enzymatic activity of rootstocks in grafted melon and watermelon plants. Front Plant Sci. 2021;13:949086.
Loewen DE, Pliakoni ED, Rivard CL. Yield and compatibility of ten tomato scion varieties grafted with ‘Maxifort’ rootstock. Urban Food Sys Symp. 2020;3:2-3.
Fallik E, Ilic Z. Grafted vegetables – The influence of rootstock and scion on postharvest quality. Folia Hortic. 2014;26(2):79-90.