Strawberry (Fragaria ananassa L.) Fruit Extract as an Antioxidant and Extracellular Matrix Protector for Anti-Photoaging
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Abstract
Skin aging, exacerbated by oxidative stress and environmental factors including UV radiation, has led to increased demand for effective anti-aging solutions. Strawberry extract, rich in antioxidants including catechins and flavonoids, has garnered attention for its potential in combating skin aging through its antioxidant and anti-inflammatory properties. This study investigates the anti-aging effects of strawberry (Fragaria ananassa L.) extract, focusing on its antioxidant capacity, inhibition of collagenase and elastase, and its impact on key aging biomarkers. Antioxidant activity was assessed using DPPH and H₂O₂ scavenging assays. The inhibition of collagenase and elastase was evaluated through enzyme activity assays. Molecular docking simulations assessed interactions between bioactive compounds in strawberry extract and anti-aging target proteins, including KEAP1, MMP1, and elastase. Strawberry extract demonstrated marked antioxidant capacity, as reflected by its IC₅₀ values of 229.91 ± 4.42 µg/mL (DPPH) and 234.08 ± 4.20 µg/mL (H₂O₂). It also showed enzyme inhibitory effects, with IC₅₀ values of 112.81 ± 1.96 µg/mL for collagenase and 28.03 ± 0.68 µg/mL for elastase. Cyanidin-3-glucoside showed binding interactions with KEAP1 and elastase (ΔG values of -9.6 and -6.4 kcal/mol), while cyanidanol interacted with MMP1 (ΔG = -9.8 kcal/mol). Strawberry extract demonstrated significant anti-aging and antioxidant effects, both in vitro and through molecular docking simulations, highlighting its potential as an potential anti-aging agent. These results highlight the potential application of strawberry extract in topical skincare products or as a dietary supplement.
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