Detection of toxic cyanobacteria and quantifi cation of microcystins in four recreational water reservoirs in Khon Kaen, Thailand
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Published:
Apr 12, 2017
Keywords:
toxic cyanobacteria Microcystis, microcystins
Main Article Content
Abstract
Toxic cyanobacterial communities of four recreational water reservoirs (Bueng Kaen Nakhon, Bueng Thung Sang, Bueng Nong Khot, and Bueng See Than) in Amphur Muang, Khon Kaen, Thailand were investigated, with particular importance given to genus Microcystis. Water samples were collected seasonally, viz. summer, monsoon, and winter for studies of diversity and density of toxic cyanobacteria, and toxin quantifi cation. The main toxic cyanobacteria present in these reservoirs were Cylindrospermopsis sp., Microcystis sp., Oscillatoria sp., and Pseudanabaena sp. The biovolume of Microcystis sp. attained a maximum of 447x103 , 132x103 , and 108x103 mm3 m-3 in the water samples collected from Bueng Nong Khot in summer, monsoon, and winter, respectively. Application of 16S rRNA analyses to Microcystis sp. isolate NK1 and NK3 show that they are most closely related to M. aeruginosa 2LT27S08 (96% similarity), and M. aeruginosa FC-070 (97% similarity), respectively. ELISA assays specifi c for hepatotoxic microcystins were carried out for detection of microcystin quantifi cation. The highest values were found in the water samples collected from Bueng Nong Khot in summer, monsoon, and winter with 1.091, 1.209, and 1.235 μg L-1, respectively.
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Somdee, T., Kaewkhiaw, K., & Somdee, A. (2017). Detection of toxic cyanobacteria and quantifi cation of microcystins in four recreational water reservoirs in Khon Kaen, Thailand. Asia-Pacific Journal of Science and Technology, 18(1), 1–8. Retrieved from https://so01.tci-thaijo.org/index.php/APST/article/view/82807
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Research Articles
References
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hypereutrophic Florida Lakes. J Phycol. 1997;
33(2): 191-5.
[26] Peerapornpisal Y, Sonthichai W, Somdee T,
Mulsin P, Rott E. Water quality and phytoplankton
in the Mae Kuang Udomtara Reservoirs Chiang
Mai, Thailand. Chiang Mai J Sci. 1999; 26(1):
25-43.
[27] Pongswat S, Thammathaworn S, Peerapornpisal Y,
Thanee N, Somsiri C. Diversity of phytoplankton
in the Rama IX Lke, a man-made lake Pathumthani
province, Thailand. Sci Asia. 2004; 30(3): 261-7.
[28]Brittain S, Mohamed ZA, Wang J, Lehmann
VKB, Carmichael WW, Rinehart KL. Isolation
and characterization of microcystins from
a River Nile strain of Oscillatoria tenuis
Agardh ex Gomont. Toxicon. 2000; 38(12): 1759-71.
[29] Oudra B, Loudiki M, Vasconcelos V, Sabour B,
Sbiyyaa B, Oufdou K. Detection and quanti
fi cation of microcystins from cyanobacterial strains
isolated from reservoirs and ponds in Morocco.
Environ Toxicol. 2002; 17(1): 32-9.
[30] Zhang Z, Schwart S, Wagner L, Miller W. A
greedy algorithm for aligning DNA sequences.
J Comput Biol. 2000: 7(1-2): 203-14
Occurrence, biosynthesis and impact on human
affairs. Mol Nutr Food Res. 2006; 50(1): 7-17.
[2] Codd GA, Morrison LF, Metcalf JS. Cyanobacterial
toxins: Risk management for health protection.
Toxicol Appl Pharmacol. 2005; 203(3): 264-72.
[3] Falconer IR. Cyanobacterial toxins of drinking
water supplies-cylindrospermopsins and
microcystins.Boca Raton: CRC Press; 2005.
[4] Carmichael WW, Beasly V, Bunner DL, Eloff
JN, Falconer I, Gorham P, et al. Naming cyclic
heptapeptide toxins of cyanobacteria (blue-green
algae). Toxicon. 1988; 26(11): 971-3.
[5] Falconer IR, Yeung DS. Cytoskeletal changes
in hepatocytes induced by Microcystis toxins
and their relation to hyperphosphorelation of
cell proteins. Chemico-Biological Interactions.
1992; 81(1-2): 181-6.
[6]MacKintosh C, Beattie KA, Klumpp S, Cohen P,
Codd GA. Cyanobacterial microcystin-LR
is a potent and specific inhibitor of protein
phosphatase 1 and 2A from both mammals and
higher plants. FEBS Lett. 1990; 264(2): 187-92.
[7]McElhiney J, Lawton LA. Detection of the
cyanobacterial hepatotoxins microcystins.
Toxicol Appl Pharmacol. 2005; 203(3): 219-30.
[8] Yoshizawa S, Matsushima R, Watanabe MF,
Harada K, Ichihara A, Carmichael WW, et al.
Inhibition of protein phosphatases by microcystins
and noduralin associated with hepatotoxicity.
J Cancer Res Clin Oncol. 1990; 116(6):609-14.
[9] Sivonen K, Jones G. Cyanobacterial toxins. In:
Chorus I, Bertram J, editors. Toxic cyanobacteria in
water. London: E and FN Spon; 1999. p. 41-111.
[10] Kuiper-Goodman T, Falconer IR, Fitzgerald J.
Human health aspects. In: Chorus I, Bertram J,
editors. Toxic cyanobacteria in water. London:
E and FN Spon; 1999. p. 113-53.
[11] Ueno Y, Nagata S, Tsutsumi T, Hasegawa A,
Watanabe MF, Park HD. Detection of microcystins,
a blue-green algal hepatotoxins, in drinking water
sample in Haimen and Fusui, endemic areas of
primary liver cancer in China, by highly sensitive
immunoassay. Carcinogenesis. 1996; 17(6):
1317-21.
[12] World Health Organization. Guidelines for
drinking-water quality. Addendum to volume 2,
health criteria and other supporting information.
2nd ed. Geneva: World Health Organization;
1998.
[13]Grosse Y, Baan R, Straif K, Secretan B,
Ghissassi FEl, Cogliano V. Carcinogenicity of
nitrate, nitrite, and cyanobacterial peptide toxins.
Lancet Oncol. 2006; 7(8): 628-9.
[14]Mahakhant A, Sano T, Ratanachot P, Tong-a-ram T,
Srivastava V, Watanabe MM. Detection of
microcystins from cyanobacterial water blooms
in Thailand freshwater. Phycol Res. 1998; 46
Suppl 2: S25-9.
[15] Peerapornpisal Y, Sonthichai W, Suckchotiratana M,
Lipigorngoson S, Ruangyuttikarn W, Ruangrit K,
et al. Survey and monitoring of toxic cyanobacteria
in water resources for water supplies and
fi sheries in Thailand. Chiang Mai J Sci. 2002; 29(2): 71-9.
[16] Prommana R, Peerapornpisal Y, Whangchai N,
Morrison LF, Metcalf JS, Ruangyuttikarn W, et al.
Microcystins in cyanobacterial blooms from two
freshwater prawn (Macrobrachium rosenbergii)
ponds in Northern Thailand. Sci Asia. 2006;
32(4): 365-70.
[17] Sungsitthisawad W, Inmuong U, Nienvitoon T,
Thiramanus T. Pollutants and heavy metals in
recreational waters in Khon Kaen City. KKU
Res J. 2000; 5(2): 70-80.Thai.
[18] Komárek J, Anagnostidis K. Cyanoprokaryota 2.
Teil/2nd part: Oscillatoriales. In: Büdel B, Gärtner G,
Krienitz L, Schagerl M, editors. Süsswasser
fl ora von Mitteleuropa, Vol 19(2). Heidelberg:
Elsevier; 2005. p. 759.
[19] Hoshaw R, Rosowski JR. Method for microscopic
algae. In: Stein JR, editor. Handbook of
phycological methods, culture methods and
growth measurements. London: Cambridge
University Press; 1973. p. 53-6.
[20]Bolch CJS, Blackburn SI. Isolation and puri
fi cation of Australian isolates of the toxic cyanobacterium
Microcystis aeruginosa Kütz. J Appl Phycol.
1996; 8(1): 5-13.
[21] Doyle JJ, Doyle JL. Isolation of plant DNA from
fresh tissue. Focus. 1990; 12(1): 13-5.
[22]Tamira K, Peterson D, Peterson N, Stecher G, Nei M,
Kumar S. MEGA5: Molecular evolutionary
genetics analysis using maximum likelihood,
evolutionary distance, and maximum parsimony
methods. Mol Biol Evol. 2011; 28(10): 2731-9.
[23] Xu Q, Chen W, Gao G. Seasonal variations in
microcystin concentrations in Lake Taihu, China.
Environ Monit Assess. 2008; 145(1-3):75-9.
[24] Robarts RD, Zohary T. Temperature effects on
photosynthetic capacity, respiration, and growth
rates of bloom-forming cyanobacteria. NZ J Mar
Freshwat Res. 1987; 21(3): 391-9.
[25] Chapman AD, Schelske CL. Recent appearance
of Cylidrospermopsis (cyanobacteria) in fi ve
hypereutrophic Florida Lakes. J Phycol. 1997;
33(2): 191-5.
[26] Peerapornpisal Y, Sonthichai W, Somdee T,
Mulsin P, Rott E. Water quality and phytoplankton
in the Mae Kuang Udomtara Reservoirs Chiang
Mai, Thailand. Chiang Mai J Sci. 1999; 26(1):
25-43.
[27] Pongswat S, Thammathaworn S, Peerapornpisal Y,
Thanee N, Somsiri C. Diversity of phytoplankton
in the Rama IX Lke, a man-made lake Pathumthani
province, Thailand. Sci Asia. 2004; 30(3): 261-7.
[28]Brittain S, Mohamed ZA, Wang J, Lehmann
VKB, Carmichael WW, Rinehart KL. Isolation
and characterization of microcystins from
a River Nile strain of Oscillatoria tenuis
Agardh ex Gomont. Toxicon. 2000; 38(12): 1759-71.
[29] Oudra B, Loudiki M, Vasconcelos V, Sabour B,
Sbiyyaa B, Oufdou K. Detection and quanti
fi cation of microcystins from cyanobacterial strains
isolated from reservoirs and ponds in Morocco.
Environ Toxicol. 2002; 17(1): 32-9.
[30] Zhang Z, Schwart S, Wagner L, Miller W. A
greedy algorithm for aligning DNA sequences.
J Comput Biol. 2000: 7(1-2): 203-14