Determining genetic structure of culture Rana rugolosa populations by RAPD analysis(Thai)

Abstract

Determining genetic structure of culture Rana rugolosa population by RAPD analysis performed with the same age group and different size of culture Rana rugulosa. Body weights of a small size group were between 50-70 g and body weights of a large size group Rana rugulosa were between 100-150 g. Fresh tissue from muscle of each Rana rugulosa was used for DNA extraction. The results of the study indicated that the concentration of extracted DNA was 55 to 3,755 ng/µL. The purity of DNA was 1.27 to 1.83. The result of DNA amplification with various primers; OPE15, OPE19, OPE20, OPY15 and OPY19, yield 434 DNA fragments. Size of DNA bands were between 300 to 1031 base pairs. The polymorphic band was 26.0 to 47.5 %. A genetic distance (D) was 0.09 to 0.64 and similarity index (S) was 0.36 to 0.91. The results of the present study indicated that all sizes of the same age population from different brood stocks were the same population. The different of growth resulted from environment or culture technique.