Pathogenicity and molecular detection of Fusarium Solani form A and B on root rot of mulberry

Abstract

Two morphological forms of Fusarium solani (Mart.) Appel & Wollenw., designated as form A (FS-A) and form B (FS-B) were isolated from mulberry roots with symptoms of root rot disease. All of FS-A and FS-B forms were pink and white colonies, respectively. The pathogenicity test of both forms on mulberry roots by detached root technique and on mulberry cutting showed infection capability of root rot at 33.8 - 85% disease severity and 30-100% disease incidence, respectively. All isolates can be recovered from brous roots, lateral roots and basal of the cutting trees at 76 - 100, 60 - 90, and 40 - 62%, respectively. The primer Fsol-F1 and Fsol-R1 showed good speci city for F. solani, at the speci c level, the 300 bp PCR product was ampli ed exclusively from both mycelium and infected mulberry roots.