Chitinase gene cloning from effective isolate of Trichoderma Harzianum for target spot resistant induction of tomato

Abstract

Chitinase gene was cloned from the effective isolate of T. harzianum for target spot resistant induction of tomato. The chitinase gene was amplified by the PCR technique. The PCR product obtained was approximately 1,200 bp. PCR product was cloned using pGEM- T vector. Results showed nucleotide sequence of that the cloned gene of T. harzianum was similar to that of T. harzianum chit-HAR2 gene for endochitinase-HAR2 (AB041752) with 99% identity and for amino acid sequence 98% was similar to that of endochitinase-HAR2 from the teleomorph of T. harzianum (Hypocrea lixii), (BAB40590) based on the GenBank database. The cloned chitinase gene obtained from this study could be constructed and transferred to tomato plants for improving disease resistance.