Extraction of bioactive compounds from luminescent mushroom (Neonothopanus nambi) and it's effect on Root-Knot Nematode (Meloidogyne incognita)

Abstract

Dry mycelia and culture filtrates from 3 isolates (PW1, PW2 and KKU) of luminescent mushroom (Neonothopanus nambi) were extracted in order to obtain bioactive compounds. Extraction using dry mycelium derived bioactive compound powder more than using culture filtrate with extraction efficiency of 8.73-12.90%. The effect ofbioactivecompound was investigatedoninfectious larvae(J2)of root-knotnematode(Meloidogyne incognita) in laboratory. The result showed that bioactive compoundat concentration500 mg/l caused100 % mortalityof J2in1 min. Concentrations of 100 and 50 mg/l affected J2 causing significantly mortalities of 100 % in 30 min and 48 hr, respectively. Effect of this bioactive compound on J2was also confirmed in screenedhouse experiment and found that concentrationsof100and500 mg/l suppressed evidentlyJ2 without root-knot symptom ontomatoplants. Root-knot score1 wasdetectableontoma to plants treated withthe compound of10 and 50 mg/l. However, it was significantly (P<0.05) from control treatment, which exhibitedroot-knot score4. This study suggests the efficiency and potential of the application of bioactive compound from N. nambi for control root-knot nematode.