Primer set test for race identification of Fusarium oxysporum f.sp. lycopersici (Thai)

Abstract

The primer set composed of uni, sp13, sp23 and sprl, which has been reported to classify the race of Fusarium oxysporum f.sp. lycopersici (Fol) was evaluated using the PCR technique on eleven Fol isolates collected in Thailand; KK1, KK2, KK3, KK4, KK5, KK6, KS, CM1, CM2, CM3 and PP1 and standard races; Fol 1, Fol 2, Fol 3N and Fol 3A. The results reveal that the primer uni gave rise to a PCR product band of 670-672 bp in all tested Fol isolates. A DNA band of 445 bp was detected in all isolates of Fol when primer sp13 was used. However, primer sp 23 showed a DNA band (approximately 518 bp) only in race 3 (Fol 3A). The primer sprl did not express any DNA band in all Fol isolates. This work suggests that the primer set could not classify races of Fol isolated in Thailand. A molecular method according to DNA technology should be developed for Fol race classification and detection for supporting phyto-sanitary programs in Thailand.