Experimental design to enhance xylanase production from Paenibacillus curdlanolyticus B6 utilizing corn cob as carbon source

Abstract

Possibility of xylanase production from Paenibacillus curdlanolyticus B6 utilizing corn cob as carbon source was studied. The results indicated that xylanase activity was found to be 2.66 U/mg. Consequently, fractional factorial design was employed to study the effects of initial pH, cultivation temperature, carbon source concentration and nitrogen source concentration on xylanase production. The results suggested that cultivation temperature and corn cob concentration were significantly affected to the yield of xylanase (p < 0.05). The parameters for enhanced xylanase production up to 450% (14.63 U/mg) were found to be initial pH of 7.0, cultivation temperature of 40 °C, corn cob and NaNO3 concentrations of 1.0 % (w/v). Therefore, it is possible to use corn cob as carbon source for the production of xylanase from P. curdlanolyticus B6.