Comparison of diversity of bacteria and actinomycetes using the culture-dependent technique and 16S rRNA clone library analysis technique (Thai)

Abstract

The diversity of bacteria and actinomycetes in pelleted organic fertilizer from a biofertilizer plant at Takam Subdistrict Administrative Organization, Hat Yai district, Songkhla province was studied by comparing the culture-dependent technique and the 16S rRNA clone library analysis technique. The results of the culturedependent method found Gram positive rod shape bacteria and thermoduric or thermophilic actinomycetes. Using the 16S rRNA clone library analysis technique, halophilic bacteria, namely Halomonas (64% of total clones) and halophilic actinomycetes, namely Dietzia (71% of total clones), were the most dominant groups which were not obtained by the culture-dependent technique. Although the results from the two techniques did not correlate, this may be due to some limitations of each technique. However, the results of the two techniques showed more diversity of bacteria and actinomycetes than only using one technique. Therefore, the results from this study can be beneficial not only for obtaining microbial biodiversity data from organic fertilizer production but also can be exploited for the development of bio-organic fertilizer production in the future.