Molecular typing of Campylobacter jejuni isolated from Mahasarakham, Khon-kaen, Roi-Ed and Nakhonphanom provinces by PCR-RAPD

Abstract

The molecular typing of 21 isolates of Campylobacter jejuni from Mahasarakham, Khon-kaen, Roi-Ed and Nakhonphanom provinces, Thailand was studied using Random Amplified Polymorphic DNA (PCR-RAPD) technique with a random primer, OPL- 07. The RAPD DNA profile showed eleven unique DNA band patterns. The C. jejuni isolates could be divived into two groups, Cluster A and Cluster B. There were 14 isolates identified as member of Cluster A. There were also two subgenotypes in Cluster A , A1 (FBRL-S14, FBRL-S15, FBRL-S22 FBRL-S26, FBRL-S29, FBRL-S35, FBRL-S41, FBRL-S57 and FBRL-S58) and A2 (FBRL-S01, FBRL-S05, FBRL-S23, FBRL-S36 and FBRL-S61). There were 6 isolates identified as Cluster B (FBRL-S20, FBRL-S25, FBRL-S37 FBRL-S70, FBRL-S71 and FBRL-S73). The results of this study demonstrated that PCR-RAPD could be used to differentiate origins of some strains of C. jejuni.