RAPD markers linked to BmNPV susceptibility in Silkworm (Bombyx mori L.) (Thai)

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Published: Apr 19, 2017
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Monthira Monthatong
Professor of Department of Biology, Faculty of Science, Khon Kaen University, Thailand
Thanes Chantes
Director of Queen Sirirkit Sericullure Center, Khon Kaen Province, Thailand
Nadda Kiatsopit
Bachelor's Degree of Department of Biology, Faculty of Science, Khon Kaen University, Thailand

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) is the significant virus causing Grassery disease in silkworm (Bombym mori Linn.). Silkworm breeding has genetically established silkworm varieties for BmNPV resistance. In this research, the DNA markers linked to the ability in BmNPV resistance were identified by using the RAPD technique. DNA samples were extracted from posterior silk glands of the fifth instars of 11 silkworm varieties with different levels of BmNPV resistance. We obtained 8 primers among 25 primers that were able to amplify 157 DNA fragments, averaging 20 DNA fragments/primer. A total of 56 fragments showed polymorphism among silkworm varieties. Interestingly, there were two fragments, size 1689 bp and 900 bp, that were specific to BmNPV susceptible silkworms.

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How to Cite
Monthatong, M., Chantes, T., & Kiatsopit, N. (2017). RAPD markers linked to BmNPV susceptibility in Silkworm (Bombyx mori L.) (Thai). Asia-Pacific Journal of Science and Technology, 14(11), 1053–1060. Retrieved from https://so01.tci-thaijo.org/index.php/APST/article/view/83595
Issue
Vol. 14 No. 11 (2009): November
Section
Research Articles

References

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