The immobilization of Tyrosinase enzyme on Polyethersulfone membrane and its applications

Abstract

On-membrane analysis of tyrosinase activity and chromatographic detection of tyrosinase inhibitor was
developed and optimized. The activity of immobilized tyrosinase was determined from the conversion of
L-dopa to melanin staining on polyethersulfone (PES) membrane. Tyrosinase could be immobilized on PES
membrane by simply using the pipetting technique. Copper sulfate was found to enhance the immobilized
tyrosinase activity. Trimethylhydroquinone (TMHQ) inhibited immobilized tyrosinase activity and was
observed as white spots in contrast to the dark background of stained melanin on the PES membrane. The sizes
of inhibitory zones were quantified by using Gel Doc. The tyrosinase inhibitory effect of TMHQ was concen-
tration dependent. TMHQ at the concentration range of 40-80g could be detected by using an optimized
on-membrane chromatographic detection of tyrosinase activity on PES membrane.