In Vitro comparative study of effective microorganisms(EMs) and wood vinegar as inhibitors for Burkholderia pseudomallei and Burkholderia thailandensis(Thai)

Abstract

Effective Microorganism (EM) fermentation at 15 days showed total microorganism counts of 1.38 x
10¹¹cfu/ml. EMs had an upper concentration level of 10%.Burkholderia pseudomallei
could not be isolated from all samples after inoculation of organisms on day 0 and a concentration upper level of 5% while pathogens could not be isolated from all samples after inoculating organisms on day 1. In addition, with EM concentration upper level of 20% B. thailandensis could not be isolated from all samples after adding organisms on day 0 while at a concentration upper level of 5%, this bacterium could not be detected after adding organisms on day 1. The pHs of EM dilutions at 0%, 5%, 10%, 20%, 40%, 50%, 70%, and 100% were
7.30, 6.33, 5.67, 5.32, 5.13, 4.74, 4.41 and 3.72, respectively. The pHs of equivalent Wood Vinegar
dilutions were 7.07, 6.15, 5.88, 5.67, 5.31, 5.09, 4.70 and 3.47, respectively. Finally, we would say that
both EMs and Wood Vinegar had a strong effect for killing B. pseudomallei and B. thailandensis
in vitro.