Detection of tick-born disease with polymerase chain reaction PCR in dairy TMZ (Thai)

Abstract

                   The objectives of this research are to compare the detection of Anaplasma marginale ,Babesia bovis and Babesia bigemina by blood smearing and PCR technique; and to compare the blood-collection location with the infection percent. All cattle used in this study have not been treated by Anti-blood parasite for at least 3 months. Blood samples from jugular vein and tail vein were investigated by blood smear and PCR technique for A. maginale,B. bovis and B. bigemina. PCR technique has higher sensitivity and accuracy compared to blood smear technique (p<0.05) for A. maginale, B. bovis and B. bigemina. In addition there were no differences of infection percentage between blood collection location (p>0.05). The result from serial dilution showed that the least amount of DNA to detect the infection were 0.1 ng/μl for A. maginale and B. bigemina and 0.7 ng/ μl for B. bovis.