Cultural condition improvement for xylanase production by Bacillus subtilis GN156

Abstract

Xylanase is important enzyme widely used in industrial process for xylose, xylitol, and
xylooligosaccharides production, which is multipurpose used in food and pharmaceutical industries. To increase the
production of xylanase from Bacillus subtilis GN156, the physical parameters were focused. By varying temperature
(25, 30 and 40 °C), shaking speed of the incubator shaker (150 and 200 rmp) and pH of culture medium (4-8), the
optimum condition for xylanase fermentation was found at pH 7, 30ºC and 200 rpm. The maximum xylanase was
1.86 folds higher than those from the production at the non-optimization condition at 37°C, 200 rpm and without
pH control. Therefore, temperature, pH and speed of the incubator shaker were the affecting factors to xylanase
production by B. subtilis GN156.